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http://www.alice.cnptia.embrapa.br/alice/handle/doc/711912Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | STEINBERG, R. S. | |
| dc.contributor.author | PEREIRA, L. | |
| dc.contributor.author | LACORTE, G. A. | |
| dc.contributor.author | PEIXOTO, M. G. C. D. | |
| dc.contributor.author | VERNEQUE, R. da S. | |
| dc.contributor.author | TEODORO, R. L. | |
| dc.contributor.author | MACHADO, M. A. | |
| dc.contributor.author | FONSECA, C. G. | |
| dc.contributor.author | CARVALHO, M. R. S. | |
| dc.date.accessioned | 2024-06-05T12:53:42Z | - |
| dc.date.available | 2024-06-05T12:53:42Z | - |
| dc.date.created | 2010-04-27 | |
| dc.date.issued | 2009 | |
| dc.identifier.citation | Journal of Dairy Science, v. 92, p. 773-776, 2009. | |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/711912 | - |
| dc.description | A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferasel (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT! gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGATI K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGATI genotyping for gene-assisted selection. | eng |
| dc.language.iso | eng | |
| dc.rights | openAccess | |
| dc.subject | Gado de leite | |
| dc.title | A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle. | |
| dc.type | Artigo de periódico | |
| dc.description.notes | Nota Técnica. | |
| riaa.ainfo.id | 711912 | |
| riaa.ainfo.lastupdate | 2024-06-05 | |
| dc.contributor.institution | UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE MINAS GERAIS; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; UNIVERSIDADE FEDERAL DE MINAS GERAIS; UNIVERSIDADE FEDERAL DE JUIZ DE FORA. | |
| Appears in Collections: | Nota Técnica/Nota científica (CNPGL)  | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| A-new-and-cost-effective-method-for-detection.pdf | 1.07 MB | Adobe PDF |  View/Open |
