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dc.contributor.authorOCHOA-CORONA, F. M.
dc.contributor.authorLOCALI-FABRIS, E. C.
dc.contributor.authorFREITAS-ASTÚA, J.
dc.date.accessioned2023-08-02T18:23:45Z-
dc.date.available2023-08-02T18:23:45Z-
dc.date.created2010-05-03
dc.date.issued2009
dc.identifier.citationPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/748515-
dc.descriptionCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.
dc.language.isoeng
dc.rightsopenAccess
dc.titleAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.
dc.typeResumo em anais e proceedings
dc.subject.thesagroBrevipalpus Phoenicis
dc.subject.thesagroFruta Cítrica
dc.subject.nalthesaurusCitrus
dc.description.notesAbstracts of presentations, Annual Meeting of the APS 2009. p. S28.
riaa.ainfo.id748515
riaa.ainfo.lastupdate2023-08-02
dc.contributor.institutionOklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF.
Aparece en las colecciones:Resumo em anais de congresso (CNPMF)

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