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dc.contributor.authorINOUE-NAGATA, A.K.
dc.contributor.authorFONSECA, M. E. N.
dc.contributor.authorLOBO, O. T. A.
dc.contributor.authorAVILA, A. C. de
dc.contributor.authorMONTE, D. C.
dc.date.accessioned2024-10-08T18:53:37Z-
dc.date.available2024-10-08T18:53:37Z-
dc.date.created2001-05-08
dc.date.issued2001
dc.identifier.citationFitopatologia Brasileira, Brasilia, v.26, n.1, p.45-52, mar. 2001.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/769749-
dc.descriptionTwo Brazilian Potato virus Y (PVY) isolates were biologically characterized as necrotic (PVY-NBR) and common (PVY-OBR) based upon symptoms on test plants. Additional characterization was performed by sequencing a cDNA corresponding to the 3’ terminal region of the viral genome. The sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (NIb) gene, 804 nt of the coat protein (CP) gene, and 328 nt (PVY-OBR) or 326 nt (PVY-NBR) of the 3’-untranslated region (UTR). Translation of the sequence resulted in one single open reading frame with part of the NIb and a CP of 267 amino acids. The two isolates shared 95.1% similarity in the CP amino acid sequence. The CP and the 3’-UTR sequence of the Brazilian isolates were compared to those of other PVY isolates previously reported and unrooted phylogenetic trees were constructed. The trees revealed a separation of two distinct clusters, one comprising most of the common strains and the other comprising the necrotic strains. PVY-OBR was clustered in the common group and PVY-NBR in the necrotic one.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectSequencia de nucleotideos
dc.subjectCaracterizacao
dc.subjectPVY
dc.subjectCharacterization
dc.subjectNucleotide sequence
dc.titleAnalysis of the nucleotide sequence os the coat protein and 3: untranslated region of two Brazilian Potato Virus Y isolate
dc.typeArtigo de periódico
dc.subject.thesagroTaxonomia
dc.subject.thesagroVírus
dc.subject.nalthesaurusPotyvirus
dc.subject.nalthesaurustaxonomy
riaa.ainfo.id769749
riaa.ainfo.lastupdate2024-10-08
dc.contributor.institutionEMBRAPA-CNPH, Brasilia, DF.
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