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dc.contributor.authorROMANO, E.
dc.contributor.authorFERREIRA, A. T.
dc.contributor.authorDUSI, A. N.
dc.contributor.authorPROITE, K.
dc.contributor.authorBUSO, J. A.
dc.contributor.authorAVILA, A. C.
dc.contributor.authorNISHIJIMA, M. L.
dc.contributor.authorNASCIMENTO, A. S.
dc.contributor.authorALMONACID-BRAVO, F.
dc.contributor.authorMENTABERRY, A.
dc.contributor.authorMONTE, D.
dc.contributor.authorCAMPOS, M. A.
dc.contributor.authorMELO, P. E.
dc.contributor.authorCATONY, M. K.
dc.contributor.authorTORRES, A. C.
dc.date.accessioned2024-10-08T20:53:40Z-
dc.date.available2024-10-08T20:53:40Z-
dc.date.created2001-08-02
dc.date.issued2001
dc.identifier.citationHorticultura Brasileira, Brasilia, v.19, n.2, p.118-122, jul. 2001.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/770257-
dc.descriptionThe coat protein (CP) gene of the potato virus Y strain “o” (PVYO) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectBatata transgenica
dc.subjectCultivar Achat
dc.subjectGene de capa proteica
dc.subjectCP
dc.subjectDistrito Federal
dc.subjectBrasil
dc.subjectTransgenic potatoes
dc.subjectDiseases
dc.subjectPVY-OBR
dc.subjectPVY-NBR
dc.subjectResistance
dc.subjectCoat protein
dc.titleExtreme resistance to two Brazilian strains of potato virus Y (PVY) in transgenic potato, cv. Achat, expressing the PVY coat protein.
dc.typeArtigo de periódico
dc.subject.thesagroCerrado
dc.subject.thesagroDoença
dc.subject.thesagroResistência
dc.subject.thesagroSolanum Tuberosum
dc.subject.thesagroVírus
dc.subject.nalthesaurusBrazil
riaa.ainfo.id770257
riaa.ainfo.lastupdate2024-10-08
dc.contributor.institutionEMBRAPA-CNPH, Brasilia, DF.
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