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dc.contributor.authorNOBILE, P. M.eng
dc.contributor.authorQUECINI, V.eng
dc.contributor.authorBAZZO, B.eng
dc.contributor.authorQUITERIO, G.eng
dc.contributor.authorMAZZAFERA, P.eng
dc.contributor.authorCOLOMBO, C. A.eng
dc.date.accessioned2019-11-02T00:37:35Z-
dc.date.available2019-11-02T00:37:35Z-
dc.date.created2010-06-14
dc.date.issued2010
dc.identifier.citationJournal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/854944-
dc.descriptionThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectFator anti-nutricionaleng
dc.subjectFerritinaeng
dc.subjectFitatoeng
dc.titleTranscriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea.eng
dc.typeArtigo de periódicoeng
dc.date.updated2019-11-02T00:37:35Z
dc.subject.thesagroCaféeng
dc.subject.thesagroBiologiaeng
dc.subject.thesagroGenéticaeng
dc.subject.thesagroFerroeng
riaa.ainfo.id854944eng
riaa.ainfo.lastupdate2019-11-01
dc.identifier.doi10.1021/jf9043088eng
dc.contributor.institutionPAULA M. NOBILE, IAC; VERA MARIA QUECINI, CNPUV; BARBARA BAZZO, IAC; GABRIELA QUITERIO, IAC; PAULO MAZZAFERA, INSTITUTO DE BIOLOGIA - UNICAMP; CARLOS A. COLOMBO, IAC.eng
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