Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/855158
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorOLIVEIRA, M. B.pt_BR
dc.contributor.authorNASCIMENTO, L. B.pt_BR
dc.contributor.authorJUNIOR, M. L.pt_BR
dc.contributor.authorPETROFEZA, S.pt_BR
dc.date.accessioned2011-04-09T14:42:05Z-
dc.date.available2011-04-09T14:42:05Z-
dc.date.created2010-06-16pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationGenetics and Molecular Research, v. 9, n. 2, p. 994-1004, 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/855158pt_BR
dc.descriptionPolygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in bean (Phaseolus vulgaris) stems infected with Sclerotinia sclerotiorum to determine whether any of them are associated with the infection process. Transcriptional analysis was carried out by means of semi-quantitative reverse transcription PCR or real-time PCR. The sspg1 gene was highly expressed during infection; sspg3 was regulated during the later phases of infection; sspg5 was more uniformly expressed during infection, whereas sspg6 was only weakly expressed. During the course of infection, Pvpgip1 transcripts were not detected at early stages, but they appeared 72 h post-inoculation. High levels of Pvpgip2 expression were observed during the initial phase of infection; the transcript peaked by 48 h post-inoculation and declined by 72 h post-inoculation. Pvpgip3 expression increased strongly at 96 h post-inoculation. Pvpgip4 was constantly present from 24 h post-inoculation until the end of the experiment. However, we detected higher levels of the Pvpgip4 transcript in the necrotic lesion area than in plants that had been mechanically wounded. Remarkably, only Pvpgip4 appeared to be moderately induced by mechanical wounding. These results provide evidence that endopolygalacturonases contribute to the infection process during host colonization by promoting the release of plant cell oligogalacturonides, which are powerful signaling molecules and may also activate plant defenses, such as polygalacturonase-inhibiting proteins.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEndopolygalacturonasept_BR
dc.subjectPolygalacturonase-inhibiting proteinpt_BR
dc.titleCharacterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroFeijãopt_BR
dc.subject.thesagroPhaseolus vulgarispt_BR
dc.subject.thesagroMofo brancopt_BR
dc.subject.thesagroDoença fúngicapt_BR
dc.subject.thesagroSclerotinia sclerotiorumpt_BR
riaa.ainfo.id855158pt_BR
riaa.ainfo.lastupdate2010-09-02pt_BR
dc.contributor.institutionM. B. OLIVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; L. B. NASCIMENTO, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; S. PETROFEZA, UNIVERSIDADE FEDERAL DE GOIÁS.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPAF)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
gmr776.pdf930,82 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace