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dc.contributor.authorLIMA, L. S.
dc.contributor.authorGRAMACHO, K. P.
dc.contributor.authorPIRES, J. L.
dc.contributor.authorCLEMENT, D.
dc.contributor.authorLOPES, U. V.
dc.contributor.authorCARELS, N.
dc.contributor.authorGESTEIRA, A. da S.
dc.contributor.authorGAIOTTO, F. A.
dc.contributor.authorCASCARDO, J. C. de MATTOS
dc.contributor.authorMICHELI, F.
dc.date.accessioned2022-08-16T13:28:17Z-
dc.date.available2022-08-16T13:28:17Z-
dc.date.created2010-07-21
dc.date.issued2010
dc.identifier.citationTree Genetics & Genomes, Heidelberg, v. p. 663–676mar. 2010.
dc.identifier.isbn1614-2950 (Online)
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/858299-
dc.descriptionIn this study, we report results of the detection and analysis of SSR markers derived of cacao?Moniliophthora perniciosa expressed sequence tags (ESTs) in relation to cacao resistance to witches? broom disease (WBD), and we compare the polymorphism of those ESTs (EST-simple sequence repeat (SSR)) with classical neutral SSR markers. A total of 3,487 ESTs was used in this investigation. SSRs were identified in 430 sequences: 277 from the resistant genotype TSH 1188 and 153 from the susceptible one Catongo, totalizing 505 EST-SSRs with three types of motifs: dinucleotides (72.1%), trinucleotides (27.3%), and tetranucleotides (0.6%). EST-SSRs were classified into 16 main categories; most of the EST-SSRs belonged to ?Unknown function? and ?No homology? categories (45.82%). A high frequency of SSRs was found in the 5?UTR and in the ORF (about 27%) and a low frequency was observed in the 3?UTR (about 8%). Forty-nine ESTSSR primers were designed and evaluated in 21 cacao accessions, 12 revealed polymorphism, having 47 alleles in total, with an average of 3.92 alleles per locus. On the other hand, the 11 genomic SSR markers revealed a total of 47 alleles, with an average of 5.22 alleles per locus. The association of EST-SSR with the genomic SSR enhanced the analysis of genetic distance among the genotypes. Among the 12 polymorphic EST-SSR markers, two were mapped on the F2 Sca 6×ICS 1 population reference for WBD resistance.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectWitches' broom disease
dc.subjectMicrosatellite
dc.subjectResistance
dc.subjectGenetic diversity
dc.subjectTheobroma cacao L
dc.titleDevelopment, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.-Moniliophthora perniciosa interaction ESTs.
dc.typeArtigo de periódico
dc.subject.thesagroCacau
dc.subject.thesagroDoença de Planta
dc.subject.thesagroMarcador Molecular
dc.subject.thesagroVassoura de Bruxa
riaa.ainfo.id858299
riaa.ainfo.lastupdate2022-08-16
dc.identifier.doi10.1007/s11295-010-0282-1
dc.contributor.institutionLÍVIA SANTOS LIMA, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRA
dc.contributor.institutionKARINA PERES GRAMACHO, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRAeng
dc.contributor.institutionJOSÉ LUIS PIRES, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRAeng
dc.contributor.institutionDIDIER CLEMENT, CENTRE DE COOPERATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LE DÉVELOPPEMENTeng
dc.contributor.institutionUILSON VANDERLEI LOPES, COMISSÃO EXECUTIVA DO PLANO DA LAVOURA CACAUEIRAeng
dc.contributor.institutionNICOLAS CARELS, UNIVERSIDADE ESTADUAL DE SANTA CRUZeng
dc.contributor.institutionABELMON DA SILVA GESTEIRA, CNPMFeng
dc.contributor.institutionFERNANDA AMATO GAIOTTO, UNIVERSIDADE ESTADUAL DE SANTA CRUZeng
dc.contributor.institutionJÚLIO CÉZAR DE MATTOS CASCARDO, UNIVERSIDADE ESTADUAL DE SANTA CRUZeng
dc.contributor.institutionFABIENNE MICHELI, CENTRE DE COOPERATION INTERNATIONALE EN RECHERCHE AGRONOMIQUE POUR LE DÉVELOPPEMENT.eng
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