Título:	Production and partial purification of an extracellular protein with antibacterial activity produced by Trichoderma harzianum.
Autoria:	PEREIRA, J. L. SILVA, T. A. P. QUIRINO, B. F. FELIX, C. R. ULHOA, C. J. NORONHA, E. F.
Afiliação:	J. L. PEREIRA, UNIVERSIDADE DE BRASÍLIA T. A. P. SILVA, UNIVERSIDADE DE BRASÍLIA BETANIA FERRAZ QUIRINO, CNPAE UNIVERSIDADE DE BRASÍLIA C. J. ULHOA, UNIVERSIDADE FEDERAL DE GOIÁS E. F. NORONHA, UNIVERSIDADE DE BRASÍLIA.
Ano de publicação:	2010
Referência:	In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR - SBBq, 39. LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010.
Páginas:	1 p.
Conteúdo:	Trichoderma is a fungal genus that comprises a set of species with biological control activity against phytopathogenic fungi. This activity can result by the production of enzymes, proteins or antibiotics with antimicrobial activity. In the present work, the antibacterial activity of T. harzianum isolates from Cerrado soil was evaluated against the phytopathogenic bacteria Xanthomonas campestris PV campestris and Ralstonia solanacearum. Antibacterial activity against these bacteria was detected to the isolate ALL-49, and this result was not detected for boiled samples. This activity is produced by the isolate after 48 hours of growth on liquid minimum medium containing Fusarium oxysporum mycelia as carbon source. Proteins, corresponding to the active fractions, with molecular masses between 45 and 60 kDa, were partially purified by chromatographic methods using Octyl sepharose and Q-sepharose columns, and their bactericidal activity was confirmed using a non-denaturing activity gel. The data show that T. harzianum has properties that make it an attractive candidate for the development of strategies to control Xanthomonas campestris PV campestris and Ralstonia solanacearum diseases.
Thesagro:	Purificação Trichoderma Harzianum
NAL Thesaurus:	Antibacterial properties Xanthomonas campestris pv. campestris
Palavras-chave:	Atividade antibacteriana
Tipo do material:	Resumo em anais e proceedings
Acesso:	openAccess
Aparece nas coleções:	Resumo em anais de congresso (CNPAE)