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dc.contributor.authorPALUAN, S. P.
dc.contributor.authorSILVA, R. B.
dc.contributor.authorCARVALHO, L. S.
dc.contributor.authorSANTOS, D. F. K.
dc.contributor.authorRAMOS, N. S. P. L.
dc.contributor.authorBERGMAN, J. C.
dc.contributor.authorNORONHA, E. F.
dc.contributor.authorKRUGER, R. H.
dc.contributor.authorQUIRINO, B. F.
dc.date.accessioned2026-01-28T14:48:45Z-
dc.date.available2026-01-28T14:48:45Z-
dc.date.created2010-08-18
dc.date.issued2010
dc.identifier.citationIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.
dc.identifier.citationLATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/860297-
dc.descriptionCarbohydrates such as cellulose and starch from plant matter are abundant and can yield high leveis of energy. However, the processes for obtaining energy from this source are expensive. Therefore, bacterial enzymes could be used as biocatalysts to transform plant matter into biofuels, such as bioethanol, in a more cost effective manner. A small insert metagenomic library of 70,000 clones was constructed with DNA from the microbial community found in Amazon soil. The library was screened for several enzymatic activities, including amylase, protease, lipase, beta-glycosidase and cellobiohydrolase. So far, 13 clones with positive enzymatic activity were isolated; of these, 12 express beta glycosidases and only 1 expresses amylase. These clones are undergoing sub-cloning so that the genes that encode the enzymes can be identified and their kinetic parameters can be determined. ln the future, these clones will also be sequenced.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectMetagenoma
dc.subjectSolo da Amazônia
dc.subjectBeta-glicosidase
dc.titleEnzymes of biotechnological interest prospected from a small insert metagenomic library constructed with Amazon soil microbial DNA.
dc.typeResumo em anais e proceedings
dc.subject.thesagroAmilase
dc.format.extent21 p.
riaa.ainfo.id860297
riaa.ainfo.lastupdate2026-01-28
dc.contributor.institutionS. P. PALUAN, UNIVERSIDADE CATÓLICA DE BRASÍLIA; R. B. SILVA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; L. S. CARVALHO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; D. F. K. SANTOS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; N. S. P. L. RAMOS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; J. C. BERGMAN, UNIVERSIDADE CATÓLICA DE BRASÍLIA; E. F. NORONHA, UNIVERSIDADE DE BRASÍLIA; R. H. KRUGER, UNIVERSIDADE DE BRASÍLIA; BETANIA FERRAZ QUIRINO, CNPAE.
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