Título:	Construction of metagenomic libraries with large inserts of DNA from the Amazonian soil microbiota and screening for enzymes with biotechnological potential.
Autor:	BERGMANN, J. C. COSTA, O. Y. A. RAMOS, N. S. P. L. FURTADO, R. S. KRUGER, R. H. QUIRINO, B. F.
Afiliación:	JESSICA C. BERGMANN, UNIVERSIDADE CATÓLICA DE BRASÍLIA; OHANA Y. A. COSTA, UNIVERSIDADE CATÓLICA DE BRASÍLIA; NÍDIA S. P. L. RAMOS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; RODRIGO S. FURTADO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; RICARDO H. KRUGER, UNIVERSIDADE DE BRASÍLIA; BETANIA FERRAZ QUIRINO, CNPAE.
Año:	2010
Referencia:	In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010.
Páginas:	p. 64-65.
Descripción:	Social, environmental and política! reasons are prompting the development of new alternative energy sources. The production of bioethanol from cellulose, the most abundant carbohydrate in nature, is a potential candidate for this purpose. Finding enzymes able to degrade the plant cell wall so that the monosaecharides are released and made available for fermentation in an efficient and economical way has been a challenge. Most enzymes used in industry today come from microorganisms cultured in the lab. Because only 1 % of the microbial diversity has been cultured in the lab and Brazil is known for the richness of its flora and fauna, if a similar biodiversity occurs at the microbial levei, there is a great unexplored potential for the discovery of novel microbial enzymes. The Amazonian soil has an extensive plant coverage, which makes it a favorable environment for the development of microbial communities. lt is known that 1 gram of soil can have the equivalent of 10 million bacteria, an essential component for the stability of the ecosystem. The metagenomic approach can be used to access the genomes and biotechnological potential of uncultivated microorganisms. The purpose of this work was to construct a metagepomic libracy and search for hydrolytic enzymes that may be used in the production of bioethanol.
Thesagro:	Biotecnologia Enzima
NAL Thesaurus:	Bioethanol Carbohydrates
Palabras clave:	Etanol lignocelulosico
Tipo de Material:	Resumo em anais e proceedings
Acceso:	openAccess
Aparece en las colecciones:	Resumo em anais de congresso (CNPAE)