Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/865496
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dc.contributor.authorZAROS, L. G.pt_BR
dc.contributor.authorCOUTINHO, L. L.pt_BR
dc.contributor.authorSIDER, L. H.pt_BR
dc.contributor.authorMEDEIROS, H. R. dept_BR
dc.contributor.authorNEVES, M. R. M. daspt_BR
dc.contributor.authorBENVENUTI, C. L.pt_BR
dc.contributor.authorNAVARRO, A. M. do C.pt_BR
dc.contributor.authorVIEIRA, L. da S.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2010-10-27pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationGenetics and Molecular Biology, v. 33, n. 3, p. 486-490, 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/865496pt_BR
dc.descriptionAbstract: Precise normalization with reference genes is necessary, in order to obtain reliable relative expression data in response to gastrointestinal nematode infection. By using sheep from temperate regions as models, three reference genes, viz., ribosomal protein LO (RPLO), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and succinate dehydrogenase complex subunit A (SDHA), were investigated in the abomasum, abomasal lymph nodes and small intestine of Brazilian Somalis sheep, either resistant or susceptible to gastrointestinal nematodes infections. Real time PCR was carried out by using SYBR Green I dye, and gene stability was tested by geNorm. RPLO was an ideal reference gene, since its expression was constant across treatments, presented lower variation, and was ranked as the most stable in abomasum and lymph node tissues. On the other hand, SDHA was the most stable in the small intestine followed by RPLO and GAPDH. These findings demonstrate the importance of correctly choosing reference genes prior to relative quantification. In addition, we determined that reference genes used in sheep from temperate regions, when properly tested, can be applied in animals from tropical regions such as the Brazilian Somalis sheep.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectPCR em tempo realpt_BR
dc.subjectNematódeo gastrintestinalpt_BR
dc.subjectRaça Somalis Brasileirapt_BR
dc.subjectControl genespt_BR
dc.subjectReal-time RT-PCRpt_BR
dc.subjectGastrointestinal diseasespt_BR
dc.subjectNematodespt_BR
dc.titleEvaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-09-23T11:11:11Zpt_BR
dc.subject.thesagroOvinopt_BR
dc.subject.thesagroParasitologiapt_BR
dc.subject.thesagroNematóidept_BR
dc.subject.thesagroDoença animalpt_BR
dc.subject.thesagroParasito de animalpt_BR
dc.subject.thesagroHelminto gastrintestinalpt_BR
dc.subject.nalthesaurusSheeppt_BR
dc.subject.nalthesaurusNematode infectionspt_BR
dc.subject.nalthesaurusHelminthspt_BR
dc.subject.nalthesaurusParasitespt_BR
dc.subject.nalthesaurusreverse transcriptase polymerase chain reactionpt_BR
dc.description.notesShort communication.pt_BR
riaa.ainfo.id865496pt_BR
riaa.ainfo.lastupdate2019-09-23 -03:00:00pt_BR
dc.identifier.doihttps://doi.org/10.1590/S1415-47572010000300018eng
dc.contributor.institutionLILIAN GIOTTO ZAROS, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTEpt_BR
dc.contributor.institutionLUIZ LEHMANN COUTINHO, ESCOLA SUPERIOR DE AGRICULTURA "LUIZ DE QUEIROZ"eng
dc.contributor.institutionLUCIA HELENA SIDER, CNPCeng
dc.contributor.institutionHENRIQUE ROCHA DE MEDEIROS, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTEeng
dc.contributor.institutionMARIA ROSALBA MOREIRA DAS NEVES, UNIVERSIDADE ESTADUAL VALE DO ACARAÚeng
dc.contributor.institutionCAMILA LOURDES BENVENUTI, UNIVERSIDADE ESTADUAL VALE DO ACARAÚeng
dc.contributor.institutionANDRINE MARIA DO CARMO NAVARRO, UNIVERSIDADE ESTADUAL VALE DO ACARAÚeng
dc.contributor.institutionLUIZ DA SILVA VIEIRA, CNPC.eng
Appears in Collections:Nota Técnica/Nota científica (CNPC)

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