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dc.contributor.authorPESSOA-FILHO, M.pt_BR
dc.contributor.authorRANGEL, P. H. N.pt_BR
dc.contributor.authorFERREIRA, M. E.pt_BR
dc.date.accessioned2011-04-09T14:44:50Z-
dc.date.available2011-04-09T14:44:50Z-
dc.date.created2010-11-16pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationBMC Plant Biology, v. 10, 127, Jun. 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/867069pt_BR
dc.descriptionBackground Breeding programs are usually reluctant to evaluate and use germplasm accessions other than the elite materials belonging to their advanced populations. The concept of core collections has been proposed to facilitate the access of potential users to samples of small sizes, representative of the genetic variability contained within the gene pool of a specific crop. The eventual large size of a core collection perpetuates the problem it was originally proposed to solve. The present study suggests that, in addition to the classic core collection concept, thematic core collections should be also developed for a specific crop, composed of a limited number of accessions, with a manageable size. Results The thematic core collection obtained meets the minimum requirements for a core sample - maintenance of at least 80% of the allelic richness of the thematic collection, with, approximately, 15% of its size. The method was compared with other methodologies based on the M strategy, and also with a core collection generated by random sampling. Higher proportions of retained alleles (in a core collection of equal size) or similar proportions of retained alleles (in a core collection of smaller size) were detected in the two methods based on the M strategy compared to the proposed methodology. Core sub-collections constructed by different methods were compared regarding the increase or maintenance of phenotypic diversity. No change on phenotypic diversity was detected by measuring the trait "Weight of 100 Seeds", for the tested sampling methods. Effects on linkage disequilibrium between unlinked microsatellite loci, due to sampling, are discussed. Conclusions Building of a thematic core collection was here defined by prior selection of accessions which are diverse for the trait of interest, and then by pairwise genetic distances, estimated by DNA polymorphism analysis at molecular marker loci. The resulting thematic core collection potentially reflects the maximum allele richness with the smallest sample size from a larger thematic collection. As an example, we used the development of a thematic core collection for drought tolerance in rice. It is expected that such thematic collections increase the use of germplasm by breeding programs and facilitate the study of the traits under consideration. The definition of a core collection to study drought resistance is a valuable contribution towards the understanding of the genetic control and the physiological mechanisms involved in water use efficiency in plants.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectVariabilidade genéticapt_BR
dc.subjectCore collectionpt_BR
dc.titleExtracting samples of high diversity from thematic collections of large gene banks using a genetic-distance based approach.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2019-08-19T11:11:11Zpt_BR
dc.subject.thesagroArrozpt_BR
dc.subject.thesagroOryza sativapt_BR
dc.subject.thesagroBanco de germoplasmapt_BR
dc.subject.thesagroBiodiversidadept_BR
dc.subject.thesagroMelhoramento Genético Vegetaleng
dc.subject.nalthesaurusGene bankspt_BR
riaa.ainfo.id867069pt_BR
riaa.ainfo.lastupdate2019-08-19 -03:00:00pt_BR
dc.identifier.doihttps://doi.org/10.1186/1471-2229-10-127eng
dc.contributor.institutionMARCO PESSOA-FILHO, UNB; PAULO HIDEO NAKANO RANGEL, CNPAF; MARCIO ELIAS FERREIRA, CENARGEN.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPAF)

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