Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/867894
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorSOUSA, T. N.pt_BR
dc.contributor.authorTARAZONA-SANTOS, E. M.pt_BR
dc.contributor.authorWILSON, D. J.pt_BR
dc.contributor.authorMADUREIRA, A. P.pt_BR
dc.contributor.authorFALCAO, P. R. K.pt_BR
dc.contributor.authorFONTES, C. J. F.pt_BR
dc.contributor.authorGIL, L. H. S.pt_BR
dc.contributor.authorFERREIRA, M. U.pt_BR
dc.contributor.authorCARVALHO, L. H.pt_BR
dc.contributor.authorBRITO, C. F. A.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2010-11-24pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationMalaria Journal, London, v. 9, n. 334, 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/867894pt_BR
dc.descriptionBackground. Plasmodium vivax malaria is a major public health challenge in Latin America, Asia and Oceania, with 130-435 million clinical cases per year worldwide. Invasion of host blood cells by P. vivax mainly depends on a type I membrane protein called Duffy binding protein (PvDBP). The erythrocyte-binding motif of PvDBP is a 170 amino-acid stretch located in its cysteine-rich region II (PvDBPII), which is the most variable segment of the protein. Methods. To test whether diversifying natural selection has shaped the nucleotide diversity of PvDBPII in Brazilian populations, this region was sequenced in 122 isolates from six different geographic areas. A Bayesian method was applied to test for the action of natural selection under a population genetic model that incorporates recombination. The analysis was integrated with a structural model of PvDBPII, and T- and B-cell epitopes were localized on the 3-D structure. Results. The results suggest that: (i) recombination plays an important role in determining the haplotype structure of PvDBPII, and (ii) PvDBPII appears to contain neutrally evolving codons as well as codons evolving under natural selection. Diversifying selection preferentially acts on sites identified as epitopes, particularly on amino acid residues 417, 419, and 424, which show strong linkage disequilibrium. Conclusions. This study shows that some polymorphisms of PvDBPII are present near the erythrocyte-binding domain and might serve to elude antibodies that inhibit cell invasion. Therefore, these polymorphisms should be taken into account when designing vaccines aimed at eliciting antibodies to inhibit erythrocyte invasion.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSeleção naturalpt_BR
dc.subjectModelagem estruturalpt_BR
dc.subjectVariabilidade genéticapt_BR
dc.subjectGenetic variabilitypt_BR
dc.subjectStructural modellingpt_BR
dc.titleGenetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-04-04T11:11:11Zpt_BR
dc.subject.thesagroPolimorfismopt_BR
dc.subject.nalthesaurusPlasmodium vivaxpt_BR
dc.subject.nalthesaurusNatural selectionpt_BR
dc.subject.nalthesaurusPolymorphismpt_BR
dc.subject.nalthesaurusmalariapt_BR
dc.format.extent212 p.pt_BR
riaa.ainfo.id867894pt_BR
riaa.ainfo.lastupdate2013-04-04pt_BR
dc.identifier.doidoi:10.1186/1475-2875-9-334pt_BR
dc.contributor.institutionTAIS N. SOUSA, CPqRR/FIOCRUZ; EDUARDO M. TARAZONA-SANTOS, ICB/UFMG; DANIEL J. WILSON, University of Chicago; ANA P. MADUREIRA, Universidade Federal de São João del Rei; PAULA REGINA KUSER FALCAO, CNPTIA; COR J. F. FONTES, UFMT; LUIZ H. S. GIL, IPEPATRO; MARCELO U. FERREIRA, USP; LUZIA H. CARVALHO, CPqRR/FIOCRUZ; CRISTIANA F. A. BRITO, CPqRR/FIOCRUZ.pt_BR
Aparece en las colecciones:Artigo em periódico indexado (CNPTIA)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
1475287593344.pdf1.01 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace