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dc.contributor.authorTIMBÓ, A. L. de O.pt_BR
dc.contributor.authorDAVIDE, L. C.pt_BR
dc.contributor.authorPINTO, J. E. B. P.pt_BR
dc.contributor.authorPEREIRA, A. V.pt_BR
dc.date.accessioned2011-04-09T16:09:06Z-
dc.date.available2011-04-09T16:09:06Z-
dc.date.created2010-12-07pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationCiência e Agrotecnologia, v. 34, n. 5, p. 1219-1223, set./out. 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/868965pt_BR
dc.descriptionThe objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied according to the hybrid, the enzymatic solution and the treatment time.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectIn vitro cultivationpt_BR
dc.subjectInterspecific hybridspt_BR
dc.titleProtoplast production from napier grass and pearl millet triploid hybrids.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroPennisetum Glaucumpt_BR
dc.subject.thesagroPennisetum Purpureumpt_BR
dc.subject.nalthesaurusforagept_BR
riaa.ainfo.id868965pt_BR
riaa.ainfo.lastupdate2010-12-07pt_BR
dc.identifier.doihttps://doi.org/10.1590/S1413-70542010000500020eng
dc.contributor.institutionANA LUIZA DE OLIVEIRA TIMBÓ, UFLA; LISETE CHAMA DAVIDE, UFLA; JOSÉ EDUARDO BRASIL PEREIRA PINTO, UFLA; ANTONIO VANDER PEREIRA, CNPGL.pt_BR
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