Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/879984
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCRAVEIRO, K. I. C.
dc.contributor.authorGOMES JÚNIOR, J. E.
dc.contributor.authorSILVA, M. C. M. da
dc.contributor.authorMACEDO, L. L. P.
dc.contributor.authorLUCENA, W. A.
dc.contributor.authorSILVA, M. S.
dc.contributor.authorSOUZA JÚNIOR, J. D. A. de
dc.contributor.authorOLIVEIRA, G. R.
dc.contributor.authorMAGALHÃES, M. T. Q. de
dc.contributor.authorSANTIAGO, A. D.
dc.contributor.authorSA, M. F. G. de
dc.date.accessioned2023-02-09T14:01:27Z-
dc.date.available2023-02-09T14:01:27Z-
dc.date.created2011-03-03
dc.date.issued2010
dc.identifier.citationJournal of Biotechnology, v. 145, n. 3, p. 215-221, 2010.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/879984-
dc.descriptionSugarcane giant borer (Telchin licus licus) is a serious sugarcane pest in Americas whose endophytic lifestyle hampers effective chemical and biological controls. Therefore, development of alternative control methods is extremely important. Envisaging development of transgenic plants resistant to this pest, we investigated the effect of the Bacillus thuringiensis Cry protein Cry1Ia12synth (truncated protein lacking C-terminus with plant codon usage) and variants against T. l. licus. cry1Ia12synth gene was used to generate mutated variants, which were screened for toxicity toward T. l. licus. For that purpose, an innovative technique combining cry gene shuffling with phage-display was used to build a combinatorial library comprising 1.97 × 105 Cry1Ia12synth variants. Screening of this library for variants binding to T. l. licus Brush Border Midgut Vesicles led to the identification of hundreds of clones, out of which 30 were randomly chosen for toxicity testing. Bioassays revealed four variants exhibiting activity against T. l. licus as compared to the non-toxic Cry1Ia12synth. Eight single substitutions sites were found in these active variants. Based on theoretical molecular modelling, the probable implications of these mutations are discussed. Therefore, we have four genes encoding Cry1Ia12synth variants active against T. l. licus promising for future development of resistant transgenic sugarcane lines.eng
dc.language.isoeng
dc.rightsopenAccess
dc.subjectTelchin licus licus
dc.titleVariant Cry1Ia toxins generated by DNA shuffling are active against sugarcane giant borer.
dc.typeArtigo de periódico
dc.subject.thesagroBacillus Thuringiensis
dc.subject.thesagroSaccharum Officinarum
dc.subject.nalthesaurusMolecular modelseng
riaa.ainfo.id879984
riaa.ainfo.lastupdate2023-02-09
dc.identifier.doihttps://doi.org/10.1016/j.jbiotec.2009.11.011eng
dc.contributor.institutionKILVIA INES CHAVES CRAVEIRO, SNT-GG
dc.contributor.institutionJOSÉ EDILSON GOMES JÚNIOR, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionMARIA CRISTINA MATTAR DA SILVA, CENARGENeng
dc.contributor.institutionLEONARDO L. P. MACEDO, UNIVERSIDADE CATÓLICA DE BRASÍLIAeng
dc.contributor.institutionWAGNER ALEXANDRE LUCENA, CNPAeng
dc.contributor.institutionMARILIA SANTOS SILVA, CPACeng
dc.contributor.institutionJOSÉ DJAIR ANTONINO DE SOUZA JÚNIOR, UNIVERSIDADE DE BRASÍLIAeng
dc.contributor.institutionGUSTAVO R. OLIVEIRA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionMARIANA T. QUEZADO DE MAGALHÃES, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionANTONIO DIAS SANTIAGO, CPATCeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGEN.eng
Appears in Collections:Artigo em periódico indexado (CENARGEN)

Files in This Item:
File Description SizeFormat 
1-s2.0-S016816560900532X-main.pdf516,46 kBAdobe PDFThumbnail
View/Open
Show simple item record

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace