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http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403Registro completo de metadados
| Campo DC | Valor | Idioma |
|---|---|---|
| dc.contributor.author | MISSIO, R. F. | pt_BR |
| dc.contributor.author | CAIXETA, E. T. | pt_BR |
| dc.contributor.author | ZAMBOLIM, E. M. | pt_BR |
| dc.contributor.author | ZAMBOLIM, L. | pt_BR |
| dc.contributor.author | SAKIYAMA, N. S. | pt_BR |
| dc.date.accessioned | 2011-04-09T22:27:59Z | - |
| dc.date.available | 2011-04-09T22:27:59Z | - |
| dc.date.created | 2011-03-10 | pt_BR |
| dc.date.issued | 2009 | pt_BR |
| dc.identifier.citation | In.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009. | pt_BR |
| dc.identifier.uri | http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403 | pt_BR |
| dc.description | With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica. | pt_BR |
| dc.language.iso | eng | eng |
| dc.rights | openAccess | eng |
| dc.subject | SSR marker | pt_BR |
| dc.subject | Coffee | pt_BR |
| dc.subject | Enriched genomic library | pt_BR |
| dc.subject | Molecular marker | pt_BR |
| dc.title | Development and validation of SSR markers for Coffea arabica L. | pt_BR |
| dc.type | Artigo de periódico | pt_BR |
| dc.date.updated | 2011-04-10T11:11:11Z | pt_BR |
| riaa.ainfo.id | 880403 | pt_BR |
| riaa.ainfo.lastupdate | 2011-03-10 | pt_BR |
| dc.contributor.institution | ROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA. | pt_BR |
| Aparece nas coleções: | Artigo em periódico indexado (SAPC)  | |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Developmentandvalidation.pdf | 194,29 kB | Adobe PDF |  Visualizar/Abrir |
