Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880488
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dc.contributor.authorRAMIRO, D.pt_BR
dc.contributor.authorJALLOUL, A.pt_BR
dc.contributor.authorPETITOT, A-S.pt_BR
dc.contributor.authorSA, M. F. G. dept_BR
dc.contributor.authorMALUF, M. P.pt_BR
dc.contributor.authorFERNANDEZ, D.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2011-03-11pt_BR
dc.date.issued2010pt_BR
dc.identifier.citationTREE GENETICS & GENOMES, Curitiba, v.6, p.767-781. 2010.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/880488pt_BR
dc.descriptionIn plants, WRKY proteins are a group of transcription factors existing as a gene superfamily that play important roles in regulation of defense response pathways. To assess the diversity of this protein family in coffee (Coffea spp.), data mining methods were used on a set of around 200,000 coffee expressed sequence tags. A total of 53 different putative WRKY genes were obtained, but only 22 unigenes encoding a protein with a WRKY domain were identified, eight of which are supported by full-length cDNA sequences. Alignment of WRKY domain sequences of the coffee unigenes and 72 Arabidopsis thaliana WRKY genes showed that the 22 coffee WRKY members were distributed among the main A. thaliana WRKY subgroups and shared conserved peptide domains. To assess the involvement of WRKY genes in coffee defense response pathways, their expression was analyzed under biotic (nematode and rust fungus infection), hormonal (salicylic acid, methyl-jasmonate), and wounding treatments, leaf senescence, and fruit development. Five members of WRKY groups IId and III were regulated only by pathogens and hormone treatments. Although a significant correlation of WRKY genes expression after MeJA and rust treatments was observed, expression of coffee genes involved in JA biosynthesis and lipoxygenase (EC 1.13.11.12) activity assays did not support the involvement of JA in the early coffee resistance responses to the rust pathogen. The five WRKY transcription factor members identified might play important roles as regulators of pathogen resistance responses and could be useful for improving coffee tolerance to various biotic stresses.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectRust diseasept_BR
dc.titleIdentification of coffee WRKY transcription factor genes and expression in resistance responses to pathogens.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-07-07T11:11:11Zpt_BR
dc.subject.thesagroCoffea Arábicapt_BR
dc.subject.nalthesaurusRegulator genespt_BR
dc.format.extent2767-781pt_BR
riaa.ainfo.id880488pt_BR
riaa.ainfo.lastupdate2011-07-07pt_BR
dc.contributor.institutionDANIEL RAMIRO, CIRAD; AIDA JALLOUL, University of Damascus; ANNE-SOPHIE PETITOT, CIRAD; MARIA FATIMA GROSSI DE SA, CENARGEN; MIRIAN PEREZ MALUF, SAPC; DIANA FERNANDEZ, CIRAD.pt_BR
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