Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/881022
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dc.contributor.authorPINTO, F. de O.pt_BR
dc.contributor.authorMALUF, M. P.pt_BR
dc.contributor.authorGUERREIRO-FILHO, O.pt_BR
dc.date.accessioned2011-04-09T22:29:21Z-
dc.date.available2011-04-09T22:29:21Z-
dc.date.created2011-03-16pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationPesquisa Agropecuária Brasileira, Brasília, v.42, n. 3, p.377-384, mar. 2007.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/881022pt_BR
dc.descriptionThe objective of this work was to identify expressed simple sequence repeats (SSR) markers associated to leaf miner resistance in coffee progenies. Identification of SSR markers was accomplished by directed searches on the Brazilian Coffee Expressed Sequence Tags (EST) database. Sequence analysis of 32 selected SSR loci showed that 65% repeats are of tetra-, 21% of tri- and 14% of dinucleotides. Also, expressed SSR are localized frequently in the 5'-UTR of gene transcript. Moreover, most of the genes containing SSR are associated with defense mechanisms. Polymorphisms were analyzed in progenies segregating for resistance to the leaf miner and corresponding to advanced generations of a Coffea arabica x Coffea racemosa hybrid. Frequency of SSR alleles was 2.1 per locus. However, no polymorphism associated with leaf miner resistance was identified. These results suggest that marker-assisted selection in coffee breeding should be performed on the initial cross, in which genetic variability is still significantpt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectmicrosatellitespt_BR
dc.subjectmolecular markerspt_BR
dc.subjectmicrossatélitespt_BR
dc.subjectmarcadores molecularespt_BR
dc.titleStudy of simple sequence repeat markers from coffee expressed sequences associated to leaf miner resistance.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-04-10T11:11:11Zpt_BR
dc.subject.thesagroLeucoptera Coffeellapt_BR
riaa.ainfo.id881022pt_BR
riaa.ainfo.lastupdate2011-03-16pt_BR
dc.contributor.institutionFERNANDA DE OLIVEIRA PINTO, IAC; MIRIAN PEREZ MALUF, SAPC; OLIVEIRO GUERREIRO-FILHO, IAC.pt_BR
Appears in Collections:Artigo em periódico indexado (SAPC)

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