Título:	Differential expression of genes in resistant versus susceptible Gyr x Holstein cattle challenged with the tick Rhipicephalus (Boophilus) microplus.
Autoria:	NASCIMENTO, C. S. do MACHADO, M. A. GUIMARÃES, S. E. F. GUIMARAES, M. F. M. PEIXOTO, J. O. FURLONG, J. PRATA, M. C. de A. VERNEQUE, R. da S. TEODORO, R. L. LOPES, P. S.
Afiliação:	CARLOS SOUZA DO NASCIMENTO, UFV; MARCO ANTONIO MACHADO, CNPGL; SIMONE ELIZA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; J. OLIVEIRA PEIXOTO, UFV; JOHN FURLONG, CNPGL; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; PAULO SAVIO LOPES, UFV.
Ano de publicação:	2010
Referência:	Genetics and Molecular Research, v. 9, n. 4, p. 1974-1979, 2010.
Conteúdo:	The bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems in tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative to control this parasite. We examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen Bos d3 (S100A7), calcium channel protein transient receptor potential vanilloid 6 (TRPV6), and the cysteine proteinase inhibitor gene (CST6). These genes were selected from cDNA libraries prepared from skin biopsies taken from resistant and susceptible Gyr x Holstein F₂ animals. These biopsies were also used to study the expression level of these genes through real-time PCR analysis. The relative expression levels of the S100A7, TPT1, TRPV6, and CST6 genes were 2.01 ± 0.6, 1.32 ± 0.9, 1.53 ± 1.2, and 2.03 ± 0.7 times higher in the susceptible group, respectively. Skin lesion tissue from the susceptible animals showed significantly more mRNA transcripts of these genes in comparison with the resistant animals (P = 0.001). However, this hypersensitivity does not seem to protect the susceptible animals against tick infestation.
Palavras-chave:	QRT-PCR Tick resistance S100A7 TPT1 TRPV6 CST6
Digital Object Identifier:	https://doi.org/10.4238/vol9-4gmr905
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CNPGL)