Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/882641
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorSTOLF-MOREIRA, R.pt_BR
dc.contributor.authorLEMOS, E. G. de M.pt_BR
dc.contributor.authorABDELNOOR, R. V.pt_BR
dc.contributor.authorBENEVENTI, M. A.pt_BR
dc.contributor.authorROLLA, A. A. P.pt_BR
dc.contributor.authorPEREIRA, S. dos S.pt_BR
dc.contributor.authorOLIVEIRA, M. C. N. dept_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.contributor.authorMARCELINO-GUIMARÃES, F. C.pt_BR
dc.date.accessioned2011-04-10T11:11:11Zpt_BR
dc.date.available2011-04-10T11:11:11Zpt_BR
dc.date.created2011-03-24pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationPesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 1, p. 58-65, jan. 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/882641pt_BR
dc.descriptionThe objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: GmB-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M), and the most stable genes, with the lowest M values, were the GmB-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectEstabilidade de expressãopt_BR
dc.subjectExpressão gênicapt_BR
dc.subjectRT-qPCRpt_BR
dc.titleIdentification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-10-14T11:11:11Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroBiotecnologiapt_BR
dc.subject.thesagroDeficiência hídricapt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusGene expressionpt_BR
dc.subject.nalthesaurusPlant-water relationspt_BR
dc.subject.nalthesaurusAgricultural biotechnologypt_BR
riaa.ainfo.id882641pt_BR
riaa.ainfo.lastupdate2011-10-14pt_BR
dc.contributor.institutionRENATA STOLF-MOREIRA, UNESP Jaboticabal; ELIANA GERTRUDES DE MACEDO LEMOS, UNESP Jaboticabal; RICARDO VILELA ABDELNOOR, CNPSO; MAGDA APARECIDA BENEVENTI, UFRGS; AMANDA ALVES PAIVA ROLLA, UEL; SELMA DOS SANTOS PEREIRA, UEL; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
46n01a08.pdf428.22 kBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace