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dc.contributor.authorEIRA, M. T. S. dapt_BR
dc.contributor.authorRIBEIRO, V. S.pt_BR
dc.contributor.authorFUZUOLI, L. C.pt_BR
dc.contributor.authorGUERREIRO FILHO, O.pt_BR
dc.contributor.authorWALTERS, C.pt_BR
dc.date.accessioned2011-04-13T11:11:11Zpt_BR
dc.date.available2011-04-13T11:11:11Zpt_BR
dc.date.created2011-04-13pt_BR
dc.date.issued2007pt_BR
dc.identifier.citationIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/885759pt_BR
dc.descriptionViability of seeds stored in genebanks must be maintained for several years or even centuries. Because of the difficulties in storing the seeds, coffee germplasm is maintained in field collections, presenting significant problems, such as land and labor costs and susceptibility to environmental hazards and pathogens. Storage of Coffea species in ex situ genebanks may help to preserve the threatened diversity of this important genus. Since 1976 the International Plant Genetic Resources Institute has considered coffee as a high priority for genetic conservation. For non-orthodox seed species, cryopreservation is the only technique available for long-term germplasm conservation. The genebank of Embrapa Genetic Resources and Biotechnology, in Brazil has now established a program to cryopreserve genetic resources of Coffea. The protocol was first determined for C. arabica and C. racemosa. Seeds were first dried to 0.20 g/g (in equilibrium with 78-80% RH). Sufficiently rapid cooling and warming was achieved in hermetically-sealed foil-laminate bags containing 10-11 g (or 50 seeds) by plunging bags directly into liquid nitrogen ? LN containers and placing bags removed from LN directly into a 40 °C bath. Successfully cryopreserved C. arabica and C. racemosa seeds showed minimal viability loss after two-year storage in liquid nitrogen. The same protocol is being adapted to other species of Coffea.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGenebankspt_BR
dc.subjectCoffee germplasmpt_BR
dc.titleCoffee seed cryopreservation: current research progress.pt_BR
dc.typeArtigo em anais e proceedingspt_BR
dc.date.updated2011-04-13T11:11:11Zpt_BR
riaa.ainfo.id885759pt_BR
riaa.ainfo.lastupdate2011-04-13pt_BR
dc.contributor.institutionMIRIAN THEREZINHA SOUZA DA EIRA, SAPC; IAC; IAC; NCGRP/USDA.pt_BR
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