Title:	Coffea expansin gene family and expansin expression during fruit maturation.
Authors:	BUDZINSKI, I. G. F. LEITE, T. F. TAKAHASHI, E. K. PEREIRA, L. F. P. VIEIRA, L. G. E.
Affiliation:	IAPAR; IAPAR; ÁGUAS CLARAS BIOTECNOLOGIA LTDA.; LUIZ FILIPE PROTASIO PEREIRA, SAPC; IAPAR.
Date Issued:	2007
Citation:	In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.
Description:	Expansins (EXP) are plant cell-wall loosing proteins involved in cell enlargement and developmental process such as organogenesis, seeds germination, cell wall dissolution and fruit ripening. Two families of EXP are known, α-expansin (EXPA), involved in the control of cell wall extension, and β-expansins (EXPB), the major allergens of grass pollen that also have cell wall-loosening activity. With the objective to study the role of EXP in coffee fruit maturation we selected EXP homologous sequences on the Brazilian Coffee Genome Project database. Full-length contigs were classified according the EXP family. Northern blots of pulp from fruits at the latest stages of maturation (22-32 weeks after flowering) showed increased transcription of one contig (CaEXP1). This higher transcription corresponds with a climacteric burst, during the expansion of pulp, with a decline in ?cherry? fruits. The transcription of CaEXP1 was also observed in roots, shoots, flower and flower buds, but not in leaves.
NAL Thesaurus:	Coffea
Keywords:	Oganogenesis
Type of Material:	Artigo em anais e proceedings
Access:	openAccess
Appears in Collections:	Artigo em anais de congresso (SAPC)