Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/894904
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dc.contributor.authorGARCIA, R. A. V.pt_BR
dc.contributor.authorRANGEL, P. N.pt_BR
dc.contributor.authorBRONDANI, C.pt_BR
dc.contributor.authorMARTINS, W. S.pt_BR
dc.contributor.authorMELO, L. C.pt_BR
dc.contributor.authorCARNEIRO, M. S.pt_BR
dc.contributor.authorBORBA, T. C. O.pt_BR
dc.contributor.authorBRONDANI, R. P. V.pt_BR
dc.date.accessioned2011-06-29T11:11:11Zpt_BR
dc.date.available2011-06-29T11:11:11Zpt_BR
dc.date.created2011-06-29pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationBMC Genetics, v. 12, n. 41, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/894904pt_BR
dc.descriptionBackground: Over recent years, a growing effort has been made to develop microsatellite markers for the genomic analysis of the common bean (Phaseolus vulgaris) to broaden the knowledge of the molecular genetic basis of this species. The availability of large sets of expressed sequence tags (ESTs) in public databases has given rise to an expedient approach for the identification of SSRs (Simple Sequence Repeats), specifically EST-derived SSRs. In the present work, a battery of new microsatellite markers was obtained from a search of the Phaseolus vulgaris EST database. The diversity, degree of transferability and polymorphism of these markers were tested. Results: From 9,583 valid ESTs, 4,764 had microsatellite motifs, from which 377 were used to design primers, and 302 (80.11%) showed good amplification quality. To analyze transferability, a group of 167 SSRs were tested, and the results showed that they were 82% transferable across at least one species. The highest amplification rates were observed between the species from the Phaseolus (63.7%), Vigna (25.9%), Glycine (19.8%), Medicago (10.2%), Dipterix (6%) and Arachis (1.8%) genera. The average PIC (Polymorphism Information Content) varied from 0.53 for genomic SSRs to 0.47 for EST-SSRs, and the average number of alleles per locus was 4 and 3, respectively. Among the 315 newly tested SSRs in the BJ (BAT93 X Jalo EEP558) population, 24% (76) were polymorphic. The integration of these segregant loci into a framework map composed of 123 previously obtained SSR markers yielded a total of 199 segregant loci, of which 182 (91.5%) were mapped to 14 linkage groups, resulting in a map length of 1,157 cM. Conclusions: A total of 302 newly developed EST-SSR markers, showing good amplification quality, are available for the genetic analysis of Phaseolus vulgaris. These markers showed satisfactory rates of transferability, especially between species that have great economic and genomic values. Their diversity was comparable to genomic SSRs, and they were incorporated in the common bean reference genetic map, which constitutes an important contribution to and advance in Phaseolus vulgaris genomic research.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectMicrossatélitept_BR
dc.subjectAnálise genéticapt_BR
dc.titleThe characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-07-25T11:11:11Zpt_BR
dc.subject.thesagroFeijãopt_BR
dc.subject.thesagroPhaseolus vulgarispt_BR
dc.subject.thesagroGenéticapt_BR
dc.subject.thesagroMarcador molecularpt_BR
riaa.ainfo.id894904pt_BR
riaa.ainfo.lastupdate2011-07-25pt_BR
dc.contributor.institutionROBERTHA A. V. GARCIA, UNIVERSIDADE FEDERAL DE GOIÁS; PRISCILA NASCIMENTO RANGEL, UNIVERSIDADE FEDERAL DE GOIÁS; CLAUDIO BRONDANI, CNPAF; WELLINGTON S. MARTINS, UNIVERSIDADE FEDERAL DE GOIÁS; LEONARDO CUNHA MELO, CNPAF; MONALISA S. CARNEIRO, UNIVERSIDADE FEDERAL DE SÃO CARLOS; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPAF)

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