Title:	Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of Mycobacterium tuberculosis.
Authors:	LIMA, A. M. de SALEM, J. I. SOUZA, J. V. B. de CORTEZ, A. C. A. CARVALHO, C. M. CHAVES, F. C. M. VEIGA JUNIOR, V. F. da
Affiliation:	Alita Moura de Lima, INPA; Júlia Ignez Salem, INPA; João Vicente Braga de Souza, INPA; Ana Claudia Alves Cortez, INPA; Clarice Maia Carvalho, CBA; FRANCISCO CELIO MAIA CHAVES, CPAA; Valdir Florêncio da Veiga Junior, UFAM.
Date Issued:	2011
Citation:	Electronic Journal of Biotechnology, v. 14, n. 4, p. 1-5, 2011.
Description:	Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from different parts of P. aduncum L. The bioassays were performed on 82 culture filtrates and 6 plant extracts and resulted in the detection of 1 culture filtrate that stimulated the growth of M. tuberculosis and 15 that inhibited microbial growth. None of the phytochemical extracts had an effect on the growth of M. tuberculosis. In conclusion, we observed that the endophytic fungi isolated from P. aduncum L. (Piperaceae) produced extracellular metabolites (present in the culture filtrate) that affect the growth of M. tuberculosis. These compounds have the potential to be used as antimicrobials or in the diagnosis of tuberculosis.
Thesagro:	Tuberculose
Keywords:	Estimulação de crescimento
ISSN:	0717-3458
DOI:	10.2225/vol14-issue4-fulltext-11
Type of Material:	Artigo de periódico
Access:	openAccess
Appears in Collections:	Artigo em periódico indexado (CPAA)