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dc.contributor.authorARANGO, R. E.
dc.contributor.authorTOGAWA, R. C.
dc.contributor.authorCARPENTIER, S. C
dc.contributor.authorROUX, N.
dc.contributor.authorHEKKERT, B. L.
dc.contributor.authorKEMA, G. H. J.
dc.contributor.authorSOUZA JUNIOR, M. T.
dc.date.accessioned2026-03-18T13:29:28Z-
dc.date.available2026-03-18T13:29:28Z-
dc.date.created2011-09-28
dc.date.issued2011
dc.identifier.citationTree Genetics & Genomes, v. 7, 2011.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/901588-
dc.descriptionBanana and plantain (Musa spp.) are grown in more than 120 countries in tropical and subtropical regions and constitute an important staple food for millions of people. A Musa acuminata ssp. malaccencis DH Pahang bacterial artificial chromosome (BAC) library (MAMB) was submitted for BAC-end sequencing. MAMB consists of 23,040 clones, with a 140-kbp average insert size, accounting for a five times coverage of the banana genome. A total of 46,080 reads were generated, and 42,750 (92.8%) high-quality sequences were obtained after trimming for vector and quality. Analysis of these data shows a GC content of 41.39%, whereas interspersed repeats comprise 32.3%. The most common repeated sequences found show homology to ribosomal RNA genes, particularly 18S rRNA, while the Ty3/gypsy type monkey retrotransposon is the most common retro element. The sequence data were used to generate a banana-specific repeat library containing 54 new repetitive elements which accounted for 11.86% of the total nucleotides. Simple sequence repeats represent 0.7% of the sequence data and allowed the identification of 2,455 potentially useful marker sites. Functional annotation identified 2,705 sequences that could code for proteins of known function. Microsynteny analysis shows a higher number of co-linear matches to Oryza sativa, in contrast to Arabidopsis thaliana. This database of BAC-end sequences is useful for the assembly of the complete banana genome sequence and is important for identification in functional genomics experiments.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectMusa acuminata spp malaccencis
dc.titleGenome-wide BAC-end sequencing of Musa acuminata DH pahang reveals further insights into the genome organization of banana.
dc.typeArtigo de periódico
dc.subject.nalthesaurusGenome
dc.subject.nalthesaurusBananas
dc.subject.nalthesaurusBacterial artificial chromosomes
riaa.ainfo.id901588
riaa.ainfo.lastupdate2026-03-18
dc.contributor.institutionRAFAEL E. ARANGO, UNALMED-CIB; ROBERTO COITI TOGAWA, CENARGEN; SEBASTIEN C. CARPENTIER, K. U. LEUVEN; NICOLAS ROUX, GLOBAL MUSA GENOMICS CONSORTIUM; BAS L. HEKKERT, PLANT RESEARCH INTERNATIONAL; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE.
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