Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902190
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dc.contributor.authorPROSDOCIMI, Ppt_BR
dc.contributor.authorBITTENCOURT, D.pt_BR
dc.contributor.authorSILVA, F. R. dapt_BR
dc.contributor.authorKIRST, M.pt_BR
dc.contributor.authorMOTA, P. C.pt_BR
dc.contributor.authorRECH, E. L.pt_BR
dc.contributor.otherFRANCISCO PROSDOCIMI, UFRJ, UCB; DANIELA MATIAS DE C BITTENCOURT, CPAA; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS KIRST, University of Florida; PAULO C. MOTA, UnB; ELIBIO LEOPOLDO RECH FILHO, CENARGEN.pt_BR
dc.date.accessioned2013-04-02T23:33:43Z-
dc.date.available2013-04-02T23:33:43Z-
dc.date.created2011-10-04pt_BR
dc.date.issued2011pt_BR
dc.identifier.other15966pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/902190pt_BR
dc.descriptionCharacterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution.pt_BR
dc.description.uribitstream/item/42811/1/spinningjournal.pone.0021634.pdfpt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.publisherPlos One, San Francisco, v. 6, n. 6, p. 1-15, June 2011.pt_BR
dc.relation.ispartofEmbrapa Informática Agropecuária - Artigo em periódico indexado (ALICE)pt_BR
dc.subjectSequencia de DNApt_BR
dc.subjectSequenciamentopt_BR
dc.subjectAranhaspt_BR
dc.titleSpinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution.pt_BR
dc.typeArtigo em periódico indexado (ALICE)pt_BR
dc.date.updated2013-04-02T23:33:43Zpt_BR
dc.subject.nalthesaurusSequence analysispt_BR
dc.subject.nalthesaurusAraneaept_BR
dc.subject.nalthesaurusTranscriptomicspt_BR
dc.ainfo.id902190pt_BR
dc.ainfo.lastupdate2013-04-02pt_BR
dc.identifier.doi10.1371/journal.pone.0021634pt_BR
Appears in Collections:Artigo em periódico indexado (CNPTIA)

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