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dc.contributor.authorREZENDE, J. C. dept_BR
dc.contributor.authorCARVALHO, C. H. S. dept_BR
dc.contributor.authorPASQUAL, M.pt_BR
dc.contributor.authorSANTOS, A. C. R.pt_BR
dc.contributor.authorCARVALHO, S. M. dept_BR
dc.date.accessioned2011-10-06T11:11:11Zpt_BR
dc.date.accessioned2011-10-06T11:11:11Zpt_BR
dc.date.available2011-10-06T11:11:11Zpt_BR
dc.date.available2011-10-06T11:11:11Zpt_BR
dc.date.created2011-10-06pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationCiência Rural, Santa Maria, v.41, n.3, p. 384-389, mar, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/902616pt_BR
dc.descriptionThree experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectGrowh regulatorspt_BR
dc.titleCalli induction in leaf explants of coffee elite genotypes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2011-10-06T11:11:11Zpt_BR
dc.subject.thesagroCoffea Arábicapt_BR
riaa.ainfo.id902616pt_BR
riaa.ainfo.lastupdate2011-10-06pt_BR
dc.contributor.institutionEPAMIG; CARLOS HENRIQUE S DE CARVALHO, SAPC; Universidade Federal de Lavras; CEIS.pt_BR
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