Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/908631
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dc.contributor.authorTETZNER, T. A. D.pt_BR
dc.contributor.authorSARAIVA, N. Z.pt_BR
dc.contributor.authorPERECIN, F.pt_BR
dc.contributor.authorNICIURA, S. C. M.pt_BR
dc.contributor.authorFERREIRA, C. R.pt_BR
dc.contributor.authorOLIVEIRA, C. S.pt_BR
dc.contributor.authorGARCIA, J. M.pt_BR
dc.date.accessioned2011-12-06T11:11:11Zpt_BR
dc.date.accessioned2011-12-06T11:11:11Zpt_BR
dc.date.available2011-12-06T11:11:11Zpt_BR
dc.date.available2011-12-06T11:11:11Zpt_BR
dc.date.created2011-12-06pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationRevista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/908631pt_BR
dc.descriptionEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.pt_BR
dc.language.isoengpt_BR
dc.rightsopenAccesspt_BR
dc.subjectBovine embryopt_BR
dc.subjectProtein sourcept_BR
dc.titleThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-11-25T11:11:11Zpt_BR
dc.subject.nalthesaurusin vitro culturept_BR
dc.subject.nalthesaurusovalbuminpt_BR
riaa.ainfo.id908631pt_BR
riaa.ainfo.lastupdate2013-11-25pt_BR
dc.identifier.doihttps://doi.org/10.1590/S1516-35982011001000010eng
dc.contributor.institutionTATIANE ALMEIDA DRUMMOND TETZNER, UNESP/JABOTICABALpt_BR
dc.contributor.institutionNAIARA ZOCCAL SARAIVA, UNESP/JABOTICABALeng
dc.contributor.institutionFELIPE PERECIN, FZEA-USP/PIRASSUNUNGAeng
dc.contributor.institutionSIMONE CRISTINA MEO NICIURA, CPPSEeng
dc.contributor.institutionCHRISTNA RAMIRES FERREIRA, UNICAMP/CAMPINASeng
dc.contributor.institutionCLARA SLADE OLIVEIRA, UNESP/JABOTICABALeng
dc.contributor.institutionJOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL.eng
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