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dc.contributor.authorMULINARI, F.
dc.contributor.authorBECKER-RITT, A. B.
dc.contributor.authorDEMARTINI, D. R.
dc.contributor.authorLIGABUE-BRAUN, R.
dc.contributor.authorSTANISÇUASKI, F.
dc.contributor.authorVERLI, H.
dc.contributor.authorFRAGOSO, R. R.
dc.contributor.authorSCHROEDER, E. K.
dc.contributor.authorCARLINI, C. R.
dc.contributor.authorSA, M. F. G. de
dc.date.accessioned2018-09-27T00:34:36Z-
dc.date.available2018-09-27T00:34:36Z-
dc.date.created2012-01-23
dc.date.issued2011
dc.identifier.citationBiochimica et Biophysica Acta, v. 1814, n. 12, p. 1758-1768, Dec. 2011.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/913253-
dc.descriptionUreases, nickel-dependent enzymes that catalyze the hydrolysis of urea into ammonia and bicarbonate, are widespread in plants, bacteria, and fungi. Previously, we cloned a cDNA encoding a Canavalia ensiformis urease isoform named JBURE-II, corresponding to a putative smaller urease protein (78 kDa) when compared to other plant ureases. Aiming to produce the recombinant protein, we obtained jbure-IIb, with different 3? and 5? ends, encoding a 90 kDa urease. Three peptides unique to the JBURE-II/-IIb protein were detected by mass spectrometry in seed extracts, indicating that jbure-II/-IIb is a functional gene. Comparative modeling indicates that JBURE-IIb urease has an overall shape almost identical to C. ensiformis major urease JBURE-I with all residues critical for urease activity. The cDNA was cloned into the pET101 vector and the recombinant protein was produced in Escherichia coli. The JBURE-IIb protein, although enzymatically inactive presumably due to the absence of Ni atoms in its active site, impaired the growth of a phytopathogenic fungus and showed entomotoxic properties, inhibiting diuresis of Rhodnius prolixus isolated Malpighian tubules, in concentrations similar to those reported for JBURE-I and canatoxin. The antifungal and entomotoxic properties of the recombinant JBURE-IIb apourease are consistent with a protective role of ureases in plants.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleCharacterization of JBURE-IIb isoform of Canavalia ensiformis (L.) DC urease.
dc.typeArtigo de periódico
dc.date.updated2018-09-27T00:34:36Zpt_BR
dc.subject.thesagroCanavalia Ensiformis
riaa.ainfo.id913253
riaa.ainfo.lastupdate2018-09-26
dc.contributor.institutionFERNANDA MULINARI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL
dc.contributor.institutionARLETE BEATRIZ BECKER-RITT, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionDIOGO RIBEIRO DEMARTINI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionRODRIGO LIGABUE-BRAUN, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionFERNANDA STANISÇUASKI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionHUGO VERLI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionRODRIGO DA ROCHA FRAGOSO, CPACeng
dc.contributor.institutionEVELYN KOECHE SCHROEDER, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionCÉLIA REGINA CARLINI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SULeng
dc.contributor.institutionMARIA FATIMA GROSSI DE SA, CENARGEN.eng
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