Por favor, use este identificador para citar o enlazar este ítem: http://www.alice.cnptia.embrapa.br/alice/handle/doc/913965
Registro completo de metadatos
Campo DCValorLengua/Idioma
dc.contributor.authorSILVA, M. R.
dc.contributor.authorGUIMARÃES, M. D. C.
dc.contributor.authorVEIGA, V. M. de O.
dc.contributor.authorMOREIRA, A. dos S.
dc.contributor.authorCOSTA, R. R. da
dc.contributor.authorABI-ZAID, K. C. F.
dc.contributor.authorROCHA, A. da S.
dc.contributor.authorSUFFYS, P. N.
dc.date.accessioned2024-02-06T16:32:57Z-
dc.date.available2024-02-06T16:32:57Z-
dc.date.created2012-01-31
dc.date.issued2011
dc.identifier.citationMemorias do Instituto Oswaldo Cruz, v. 106, n. 1, p. 9-15, 2011.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/913965-
dc.descriptionA cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectOxyR pseudogene
dc.subjectSputum smear
dc.subjectMolecular sequence data
dc.titleIdentification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil.
dc.typeArtigo de periódico
dc.subject.thesagroMycobacterium Bovis
dc.subject.nalthesaurusMycobacterium tuberculosis
dc.subject.nalthesauruspolymerase chain reaction
riaa.ainfo.id913965
riaa.ainfo.lastupdate2024-02-05
dc.identifier.doihttps://doi.org/10.1590/S0074-02762011000100002
dc.contributor.institutionMARCIO ROBERTO SILVA, CNPGL; MARK DREW CROSLAND GUIMARÃES, UFMG; VANIA MARIA DE OLIVEIRA VEIGA, CNPGL; ALINE DOS SANTOS MOREIRA, Laboratório de Genômica Funcional e Bioinformática; RONALDO RODRIGUES DA COSTA, UFJF; FHEMIG; KELLY CRISTINA FERREIRA ABI-ZAID, FHEMIG; ADALGIZA DA SILVA ROCHA, FIOCRUZ; PHILIP NOEL SUFFYS, FIOCRUZ.
Aparece en las colecciones:Artigo em periódico indexado (CNPGL)

Ficheros en este ítem:
Fichero Descripción TamañoFormato 
Artigo-meta-2011-MInstOC-Marcio-2.pdf631.6 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro sencillo del ítem

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace