Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.

SANTANA, J. R. F. de; PAIVA, R.; SOUZA, A. V. de; OLIVEIRA, L. M. de

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	SANTANA, J. R. F. de	pt_BR
dc.contributor.author	PAIVA, R.	pt_BR
dc.contributor.author	SOUZA, A. V. de	pt_BR
dc.contributor.author	OLIVEIRA, L. M. de	pt_BR
dc.date.accessioned	2012-02-03T11:11:11Z	pt_BR
dc.date.available	2012-02-03T11:11:11Z	pt_BR
dc.date.available	2012-02-03T11:11:11Z	pt_BR
dc.date.created	2012-02-03	pt_BR
dc.date.issued	2011	pt_BR
dc.identifier.citation	Ciência e Agrotecnologia, v. 35, n. 5, p. 916-923, set./out. 2011.	pt_BR
dc.identifier.uri	http://www.alice.cnptia.embrapa.br/alice/handle/doc/914333	pt_BR
dc.description	The present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as “araticum”. Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoa	eng
dc.language.iso	eng	eng
dc.rights	openAccess	eng
dc.subject	Tampão de algodão	pt_BR
dc.subject	Abscisão foliar	pt_BR
dc.subject	Cultivo in vitro	eng
dc.title	Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.	pt_BR
dc.type	Artigo de periódico	pt_BR
dc.date.updated	2012-02-06T11:11:11Z	pt_BR
dc.subject.thesagro	Araticum	pt_BR
dc.subject.thesagro	Annona glabra	pt_BR
dc.subject.thesagro	Fruta Tropical	pt_BR
dc.subject.nalthesaurus	Fruits	eng
dc.subject.nalthesaurus	Leaf abscission	eng
riaa.ainfo.id	914333	pt_BR
riaa.ainfo.lastupdate	2012-02-06	pt_BR
dc.contributor.institution	JOSÉ RANIERE FERREIRA DE SANTANA; RENATO PAIVA; ANA VALERIA DE SOUZA, CPATSA; LENALDO MUNIZ DE OLIVEIRA.	pt_BR
Aparece en las colecciones:	Artigo em periódico indexado (CPATSA)

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