Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/916042
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dc.contributor.authorPOLIZEL, A. M.pt_BR
dc.contributor.authorMEDRI, M. E.pt_BR
dc.contributor.authorNAKASHIMA, K.pt_BR
dc.contributor.authorYAMANAKA, N.pt_BR
dc.contributor.authorFARIAS, J. R. B.pt_BR
dc.contributor.authorOLIVEIRA, M. C. N. dept_BR
dc.contributor.authorMARIN, S. R. R.pt_BR
dc.contributor.authorABDELNOOR, R. V.pt_BR
dc.contributor.authorMARCELINO, F. C.pt_BR
dc.contributor.authorFUGANTI, R.pt_BR
dc.contributor.authorRODRIGUES, F. A.pt_BR
dc.contributor.authorSTOLF-MOREIRA, R.pt_BR
dc.contributor.authorBENEVENTI, M. A.pt_BR
dc.contributor.authorROLLA, A. A. P.pt_BR
dc.contributor.authorNEUMAIER, N.pt_BR
dc.contributor.authorYAMAGUCHI-SHINOZAKI, K.pt_BR
dc.contributor.authorCARVALHO, J. F. C.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.date.accessioned2017-07-13T18:48:17Z-
dc.date.available2017-07-13T18:48:17Z-
dc.date.created2012-02-17pt_BR
dc.date.issued2011pt_BR
dc.identifier.citationGenetics and Molecular Research, Ribeirão Preto, v. 10, n. 4, p. 3641-3656, 2011.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/916042pt_BR
dc.descriptionWe evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMolecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-13T18:48:17Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroResistência a secapt_BR
dc.subject.thesagroFisiologia vegetalpt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusDrought tolerancept_BR
dc.subject.nalthesaurusPlant physiologypt_BR
riaa.ainfo.id916042pt_BR
riaa.ainfo.lastupdate2017-07-13pt_BR
dc.identifier.doihttp://dx.doi.org/10.4238/2011.October.21.4pt_BR
dc.contributor.institutionUEL; UEL; JIRCAS; JIRCAS; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; RENATA FUGANTI, CNPSo; CNPSo; RENATA STOLF MOREIRA, CNPSo; UFRGS; AMANDA A. P. ROLLA, UEL; NORMAN NEUMAIER, CNPSO; JIRCAS; CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI.pt_BR
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