Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs.

BRITTO, F. B.; D. S. F. MENDES; OGAWA, M.; CINTRA, I. H. A.; DINIZ, F. M.

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Campo DC	Valor	Idioma
dc.contributor.author	BRITTO, F. B.	pt_BR
dc.contributor.author	D. S. F. MENDES	pt_BR
dc.contributor.author	OGAWA, M.	pt_BR
dc.contributor.author	CINTRA, I. H. A.	pt_BR
dc.contributor.author	DINIZ, F. M.	pt_BR
dc.date.accessioned	2012-03-20T11:11:11Z	pt_BR
dc.date.accessioned	2012-03-20T11:11:11Z	pt_BR
dc.date.available	2012-03-20T11:11:11Z	pt_BR
dc.date.available	2012-03-20T11:11:11Z	pt_BR
dc.date.created	2012-03-20	pt_BR
dc.date.issued	2011	pt_BR
dc.identifier.citation	Genetics and Molecular Research, Ribeirão Preto, v. 10, n. 4, p. 4084-4092, 2011.	pt_BR
dc.identifier.issn	1676-5680	pt_BR
dc.identifier.uri	http://www.alice.cnptia.embrapa.br/alice/handle/doc/919671	pt_BR
dc.description	We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of each locus. A total of 78 loci were amplified in 76 samples; high polymorphism rates were detected in the entire sample (80.8%) and within each locality (73.5-79.5%). Analysis of molecular variance demonstrates significant differences between localities (P < 0.001); however, the ΦST value (0.078) indicates a low level of genetic differentiation, which suggests that U. cordatus larvae can spread over large distances. The variation was distributed among the samples, and most of it was attributed to differences among individuals within localities. Cluster analysis, based on the Jaccard similarity coefficient, and the Mantel test gave similar results to the analysis of molecular variance data. Despite the low level of population structuring, these markers could be used for studying U. cordatus diversity, due to the high level of polymorphism.	pt_BR
dc.language.iso	eng	eng
dc.rights	openAccess	eng
dc.subject	Diversidade	pt_BR
dc.title	Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs.	pt_BR
dc.type	Artigo de periódico	pt_BR
dc.date.updated	2012-03-22T11:11:11Z	pt_BR
dc.subject.thesagro	Caranguejo	pt_BR
dc.subject.thesagro	Marcador Molecular	pt_BR
riaa.ainfo.id	919671	pt_BR
riaa.ainfo.lastupdate	2012-03-22	pt_BR
dc.contributor.institution	FABIO BARROS BRITTO, UFPI; MENDES, D. S. F., CPAMN; M. OGAWA, UFCE; ISRAEL HIDENBURGO ANICETO CINTRA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; FABIO MENDONCA DINIZ, CPAMN.	pt_BR
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