Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/919948
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorFONSECA JÚNIOR, A. A.
dc.contributor.authorMAGALHÃES, C. G.
dc.contributor.authorSALES, E. B.
dc.contributor.authorD'AMBROS, R. M.
dc.contributor.authorZANELLA, J. R. C.
dc.contributor.authorHEINEMANN, M. B.
dc.contributor.authorLEITE, R. C.
dc.contributor.authorREIS, J. K. P. dos
dc.date.accessioned2026-08-14T23:40:00Z-
dc.date.available2026-08-14T23:40:00Z-
dc.date.created2012-03-22
dc.date.issued2011
dc.identifier.citationISRN Microbiology, v. 2011. Doi:10.5402/2011/458294.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/919948-
dc.descriptionSuid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
dc.language.isoeng
dc.rightsopenAccess
dc.subjectPCR
dc.titleGenotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis.
dc.typeArtigo de periódico
dc.subject.thesagroGenótipo
dc.subject.thesagroVírus
dc.description.notesProjeto/Plano de Ação: 03.09.00.046.
riaa.ainfo.id919948
riaa.ainfo.lastupdate2023-10-20
dc.contributor.institutionANTONIO AUGUSTO FONSECA JÚNIOR, LANAGRO; CRISTINA GONÇALVES MAGALHÃES, LANAGRO; ERICA BRAVO SALES, LANAGRO; REGIA MARIA D'AMBROS, CEDISA; JANICE REIS CIACCI ZANELLA, CNPSA; MARCOS BRYAN HEINEMANN, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JENNER KARLISSON PIMENTA DOS REIS, UFMG.
Aparece nas coleções:Artigo em periódico indexado (CNPSA)

Arquivos associados a este item:
Arquivo TamanhoFormato 
genotyping-of-the-pseudorabies.pdf681,45 kBAdobe PDFVisualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace