Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/926591
Registro completo de metadados
Campo DCValorIdioma
dc.contributor.authorGUIMARÃES-DIAS, F.pt_BR
dc.contributor.authorNEVES-BORGES, A. C.pt_BR
dc.contributor.authorVIANA, A. A. B.pt_BR
dc.contributor.authorMESQUITA, R. O.pt_BR
dc.contributor.authorROMANO, E.pt_BR
dc.contributor.authorGROSSI-DE-SÁ, M. de F.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.contributor.authorLOUREIRO, M. E.pt_BR
dc.contributor.authorALVES-FERREIRA, M.pt_BR
dc.date.accessioned2012-06-18T11:11:11Zpt_BR
dc.date.available2012-06-18T11:11:11Zpt_BR
dc.date.created2012-06-18pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationGenetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 222-232, May 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/926591pt_BR
dc.descriptionMetabolomics analysis of wild type Arabidopsis thaliana plants, under control and drought stress conditions revealed several metabolic pathways that are induced under water deficit. The metabolic response to drought stress is also associated with ABA dependent and independent pathways, allowing a better understanding of the molecular mechanisms in this model plant. Through combining an in silico approach and gene expression analysis by quantitative real-time PCR, the present work aims at identifying genes of soybean metabolic pathways potentially associated with water deficit. Digital expression patterns of Arabidopsis genes, which were selected based on the basis of literature reports, were evaluated under drought stress condition by Genevestigator. Genes that showed strong induction under drought stress were selected and used as bait to identify orthologs in the soybean genome. This allowed us to select 354 genes of putative soybean orthologs of 79 Arabidopsis genes belonging to 38 distinct metabolic pathways. The expression pattern of the selected genes was verified in the subtractive libraries available in the GENOSOJA project. Subsequently, 13 genes from different metabolic pathways were selected for validation by qPCR experiments. The expression of six genes was validated in plants undergoing drought stress in both pot-based and hydroponic cultivation systems. The results suggest that the metabolic response to drought stress is conserved in Arabidopsis and soybean plants.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBioinformáticapt_BR
dc.subjectQPCRpt_BR
dc.titleExpression analysis in response to drought stress in soybean: shedding light on the regulation of metabolic pathway genes.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2012-07-17T11:11:11Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroResistência a secapt_BR
dc.subject.thesagroGenept_BR
dc.subject.thesagroGenomapt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusBioinformaticspt_BR
dc.subject.nalthesaurusBiochemical pathwayspt_BR
dc.subject.nalthesaurusDrought tolerancept_BR
dc.subject.nalthesaurusGenespt_BR
dc.subject.nalthesaurusGenomept_BR
riaa.ainfo.id926591pt_BR
riaa.ainfo.lastupdate2012-07-17pt_BR
dc.contributor.institutionFÁBIA GUIMARÃES DIAS, UFRJ; ANNA CRISTINA NEVES-BORGES, UNIRIO; ANTONIO AMERICO BARBOSA VIANA, CENARGEN; ROSILENE OLIVEIRA MESQUITA, UFV; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ALEXANDRE LIMA NEPOMUCENO, SRI; MARCELO EHLERS LOUREIRO, UFV; MÁRCIO ALVES-FERREIRA, UFRJ.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
gmb.expression.anal.v35n1s.222232.2012.pdf1,55 MBAdobe PDFThumbnail
Visualizar/Abrir
Mostrar registro simples do item Visualizar estatísticas

FacebookTwitterDeliciousLinkedInGoogle BookmarksMySpace