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dc.contributor.authorRODRIGUES, F. A.pt_BR
dc.contributor.authorMARCOLINO-GOMES, J.pt_BR
dc.contributor.authorCARVALHO, J. de F. C.pt_BR
dc.contributor.authorNASCIMENTO, L. C. dopt_BR
dc.contributor.authorNEUMAIER, N.pt_BR
dc.contributor.authorFARIAS, J. R. B.pt_BR
dc.contributor.authorCARAZZOLLE, M. F.pt_BR
dc.contributor.authorMARCELINO, F. C.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.date.accessioned2012-06-18T11:11:11Zpt_BR
dc.date.available2012-06-18T11:11:11Zpt_BR
dc.date.created2012-06-18pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationGenetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/926619pt_BR
dc.descriptionSoybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes expressed during the early response to water deficit in roots and leaves. These genes were compared among the lines to assess probable differences in the plant transcriptomes. In general, similar biochemical processes were predominant in both cultivars; however, there were more considerable differences between roots and leaves of Embrapa 48. Moreover, we present here a fast, clean and straightforward method to obtain drought-stressed root tissues and a large enriched collection of transcripts expressed by soybean plants under water deficit that can be useful for further studies towards the understanding of plant responses to stress.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleSubtractive libraries for prospecting differentially expressed genes in the soybean under water deficit.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2012-07-24T11:11:11Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroGenomapt_BR
dc.subject.thesagroGenept_BR
dc.subject.thesagroDeficiência hídricapt_BR
dc.subject.thesagroRelação água-plantapt_BR
dc.subject.thesagroSecapt_BR
dc.subject.thesagroEstiagempt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusGene expressionpt_BR
dc.subject.nalthesaurusDroughtpt_BR
riaa.ainfo.id926619pt_BR
riaa.ainfo.lastupdate2012-07-24pt_BR
dc.contributor.institutionFABIANA APARECIDA RODRIGUES, CNPSo; JULIANA MARCOLINO-GOMES, CNPSo; JOSIRLEI DE FÁTIMA CORRÊA CARVALHO, CARVALHO , J. de F. C.; LEANDRO COSTA DO NASCIMENTO, UNICAMP; NORMAN NEUMAIER, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARCELO FALSARELLA CARAZZOLLE, UNICAMP; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

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