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dc.contributor.authorOBREGÓN, D.pt_BR
dc.contributor.authorOLIVEIRA, M. C. de S.pt_BR
dc.contributor.authorTIZIOTO, P. C.pt_BR
dc.contributor.authorFUNNES, M. E.pt_BR
dc.contributor.authorMARTÍNEZ, S.pt_BR
dc.contributor.authorROQUE, E.pt_BR
dc.contributor.authorFONSECA, A. H.pt_BR
dc.contributor.authorCORONA, B.pt_BR
dc.date.accessioned2012-07-04T11:11:11Zpt_BR
dc.date.available2012-07-04T11:11:11Zpt_BR
dc.date.created2012-07-04pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationRevista de Salud Animal, v. 34, n. 2, p. 1-7, mayo-ago. 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/927636pt_BR
dc.descriptionThe objective of this work was to determine if water buffaloes from the western region of Cuba are carrier of Babesia bovis infection, using a nested PCR (nPCR) test. Two hundred twenty two DNA samples, extracted from buffalo blood at different ages, from six farms in the western region of Cuba were processed. Visualization of blood smear in the optical microcopy was previously carried out. The results showed that all the samples were negative to blood smear; however, 94 (42.1%) samples were positive in the nPCR, visualizing bands of 298 pb. Infection was present in animals from all farms and categories studied. A positive nPCR sample was sequenced and such sequence was deposited in the GenBank, comparing it with others available in diverse databases. Its high identity was verified with several geographical isolates of B. bovis. It is concluded that water buffaloes in the western region of Cuba are carrier of Babesia bovis infection.spa
dc.language.isospapt_BR
dc.rightsopenAccesspt_BR
dc.subjectPCRnpt_BR
dc.subjectRap1pt_BR
dc.titleDiagnóstico de Babesia bovis en búfalos de la región occidental de Cuba através de un ensayo de nPCR.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2016-03-01T11:11:11Zpt_BR
dc.subject.thesagroBabesia Bovispt_BR
riaa.ainfo.id927636pt_BR
riaa.ainfo.lastupdate2016-03-01pt_BR
dc.contributor.institutionD. OBREGÓN, Universidad Agraria de La Habanapt_BR
dc.contributor.institutionMARCIA CRISTINA DE SENA OLIVEIRA, CPPSEspa
dc.contributor.institutionPOLYANA CRISTINE TIZIOTO, UFSCar/SÃO CARLOS, SPspa
dc.contributor.institutionMARIBEL ELIZABETH FUNNES, UFSCar/SÃO CARLOS, SPspa
dc.contributor.institutionSIOMARA MARTÍNEZ, CENSA/MAYABEQUEspa
dc.contributor.institutionE. ROQUE, UNIVERSIDAD AGRARIA DE LA HABANAspa
dc.contributor.institutionA. HENRIQUE FONSECA, UFRRJ/RIO DE JANEIROspa
dc.contributor.institutionBELKIS CORONA, CENSA/MAYABEQUE.spa
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