Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940202
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dc.contributor.authorPROTASIO, A. V.
dc.contributor.authorTSAI, I. J.
dc.contributor.authorBABBAGE, A.
dc.contributor.authorNICHOL, S.
dc.contributor.authorHUNT, M.
dc.contributor.authorASLETT, M. A.
dc.contributor.authorSILVA, N. de
dc.contributor.authorVELARDE, G. S.
dc.contributor.authorANDERSON, T. J. C.
dc.contributor.authorCLARK, R. C.
dc.contributor.authorDAVIDSON, C.
dc.contributor.authorDILLON, G. P.
dc.contributor.authorHOLROYD, N. E.
dc.contributor.authorLOVERDE, P. T.
dc.contributor.authorLLOYD, C.
dc.contributor.authorMCQUILLAN, J.
dc.contributor.authorOLIVEIRA, G.
dc.contributor.authorOTTO, T. D.
dc.contributor.authorPARKER-MANUEL, S. J.
dc.contributor.authorQUAIL, M. A.
dc.contributor.authorWILSON, R. A.
dc.contributor.authorZERLOTINI, A.
dc.contributor.authorDUNNE, D. W.
dc.contributor.authorBERRIMAN, M.
dc.date.accessioned2018-05-11T00:37:25Z-
dc.date.available2018-05-11T00:37:25Z-
dc.date.created2012-11-21
dc.date.issued2012
dc.identifier.citationPLOS Neglected Tropical Diseases, v. 6, n. 1, p. 1-13, Jan. 2012.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/940202-
dc.descriptionSchistosomiasis is one of the most prevalent parasitic diseases, affecting millions of people in developing countries. Amongst the human-infective species, Schistosoma mansoni is also the most commonly used in the laboratory and here we present the systematic improvement of its draft genome. We used Sanger capillary and deep-coverage Illumina sequencing from clonal worms to upgrade the highly fragmented draft 380 Mb genome to one with only 885 scaffolds and more than 81% of the bases organised into chromosomes. We have also used transcriptome sequencing (RNA-seq) from four time points in the parasite?s life cycle to refine gene predictions and profile their expression. More than 45% of predicted genes have been extensively modified and the total number has been reduced from 11,807 to 10,852. Using the new version of the genome, we identified trans-splicing events occurring in at least 11% of genes and identified clear cases where it is used to resolve polycistronic transcripts. We have produced a high-resolution map of temporal changes in expression for 9,535 genes, covering an unprecedented dynamic range for this organism. All of these data have been consolidated into a searchable format within the GeneDB (www.genedb.org) and SchistoDB (www.schistodb.net) databases. With further transcriptional profiling and genome sequencing increasingly accessible, the upgraded genome will form a fundamental dataset to underpin further advances in schistosome research.
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleA systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.
dc.typeArtigo de periódico
dc.date.updated2020-04-14T11:11:11Zpt_BR
dc.subject.nalthesaurusSchistosoma
riaa.ainfo.id940202
riaa.ainfo.lastupdate2020-04-14 -03:00:00
dc.identifier.doi10.1371/journal.pntd.0001455eng
dc.contributor.institutionANNA V. PROTASIO, Wellcome Trust Sanger Institute
dc.contributor.institutionISHENG J. TSAI, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionANNE BABBAGE, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionSARAH NICHOL, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionMARTIN HUNT, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionMARTIN A. ASLETT, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionNISHADI DE SILVA, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionGILES S. VELARDE, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionTIM J. C. ANDERSON, Texas Biomedical Research Instituteeng
dc.contributor.institutionRICHARD C. CLARK, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionCLAIRE DAVIDSON, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionGARY P. DILLON, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionNANCY E. HOLROYD, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionPHILIP T. LOVERDE, University of Texas Health Science Centereng
dc.contributor.institutionCHRISTINE LLOYD, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionJACQUELLINE MCQUILLAN, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionGUILHERME OLIVEIRA, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruzeng
dc.contributor.institutionTHOMAS D. OTTO, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionSOPHIA J. PARKER-MANUEL, University of Yorkeng
dc.contributor.institutionMICHAEL A. QUAIL, Wellcome Trust Sanger Instituteeng
dc.contributor.institutionR. ALAN WILSON, University of Yorkeng
dc.contributor.institutionADHEMAR ZERLOTINI NETO, CNPTIAeng
dc.contributor.institutionDAVID W. DUNNE, University of Cambridgeeng
dc.contributor.institutionMATTHEW BERRIMAN, Wellcome Trust Sanger Institute.eng
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