Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940203
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dc.contributor.authorD'AFONSECA, V.eng
dc.contributor.authorSOARES, S. C.eng
dc.contributor.authorALI, A.eng
dc.contributor.authorSANTOS, A. R.eng
dc.contributor.authorPINTO, A. C.eng
dc.contributor.authorMAGALHÃES, A. A. C.eng
dc.contributor.authorFARIA, C. de J.eng
dc.contributor.authorBARBOSA, E.eng
dc.contributor.authorGUIMARÃES, L. C.eng
dc.contributor.authorESLABÃO, M.eng
dc.contributor.authorALMEIDA, S. S.eng
dc.contributor.authorABREU, V. A. C.eng
dc.contributor.authorZERLOTINI, A.eng
dc.contributor.authorCARNEIRO, A. R.eng
dc.contributor.authorCERDEIRA, L. T.eng
dc.contributor.authorRAMOS, R. T. J.eng
dc.contributor.authorHIRATA JÚNIOR, R.eng
dc.contributor.authorMATTOS-GUARALDI, A. L.eng
dc.contributor.authorTROST, E.eng
dc.contributor.authorTAUCH, A.eng
dc.contributor.authorSILVA, A.eng
dc.contributor.authorSCHNEIDER, M. P.eng
dc.contributor.authorMIYOSHI, A.eng
dc.contributor.authorAZEVEDO, V.eng
dc.date.accessioned2020-04-15T00:58:50Z-
dc.date.available2020-04-15T00:58:50Z-
dc.date.created2012-11-21
dc.date.issued2012
dc.identifier.citationOpen Access Bioinformatics, v. 4, p. 1-13, 2012.eng
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/940203-
dc.descriptionBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl.eng
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectBioinformáticaeng
dc.titleReannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria.eng
dc.typeArtigo de periódicoeng
dc.date.updated2020-04-15T00:58:50Z
dc.subject.thesagroGenomaeng
dc.subject.thesagroPatogenicidadeeng
dc.subject.nalthesaurusBioinformaticseng
dc.subject.nalthesaurusCorynebacterium diphtheriaeeng
dc.subject.nalthesaurusGenomeeng
dc.subject.nalthesaurusPathogenicity islandseng
riaa.ainfo.id940203eng
riaa.ainfo.lastupdate2020-04-14
dc.contributor.institutionVÍVIAN D’AFONSECA, UFMGeng
dc.contributor.institutionSIOMAR C. SOARES, UFMGeng
dc.contributor.institutionAMJAD ALI, UFMGeng
dc.contributor.institutionANDERSON R. SANTOS, UFMGeng
dc.contributor.institutionANNE C. PINTO, UFMGeng
dc.contributor.institutionARYANE A. C. MAGALHÃES, UFMGeng
dc.contributor.institutionCÁSSIO DE JESUS FARIA, UFMGeng
dc.contributor.institutionEUDES BARBOSA, UFMGeng
dc.contributor.institutionLUIS C. GUIMARÃES, UFMGeng
dc.contributor.institutionMARCUS ESLABÃO, UFPeleng
dc.contributor.institutionSINTIA S. ALMEIDA, UFMGeng
dc.contributor.institutionVINICIUS A. C. ABREU, UFMGeng
dc.contributor.institutionADHEMAR ZERLOTINI NETO, CNPTIAeng
dc.contributor.institutionADRIANA R. CARNEIRO, UFPAeng
dc.contributor.institutionLOUISE T. CERDEIRA, UFPAeng
dc.contributor.institutionROMMEL T. J. RAMOS, UFPAeng
dc.contributor.institutionRAPHAEL HIRATA JÚNIOR, UERJeng
dc.contributor.institutionANA L. MATTOS-GUARALDI, UERJeng
dc.contributor.institutionEVA TROST, Bielefeld Universityeng
dc.contributor.institutionANDREAS TAUCH, Bielefeld Universityeng
dc.contributor.institutionARTUR SILVA, UFPAeng
dc.contributor.institutionMARIA P. SCHNEIDER, UFPAeng
dc.contributor.institutionANDERSON MIYOSHI, UFMGeng
dc.contributor.institutionVASCO AZEVEDO, UFMG.eng
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