Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/941757
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dc.contributor.authorDROVAL, A. A.pt_BR
dc.contributor.authorBINNECK, E.pt_BR
dc.contributor.authorMARIN, S. R. R.pt_BR
dc.contributor.authorPAIÃO, F. G.pt_BR
dc.contributor.authorOBA, A.pt_BR
dc.contributor.authorNEPOMUCENO, A. L.pt_BR
dc.contributor.authorSHIMOKOMAKI, M.pt_BR
dc.date.accessioned2017-07-13T18:48:40Z-
dc.date.available2017-07-13T18:48:40Z-
dc.date.created2012-12-07pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationGenetics and Molecular Research, Ribeirão Preto, v. 11, n. 2, p. 821-829, 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/941757pt_BR
dc.descriptionSome genes affect meat quality in chickens. We looked for polymorphisms in the Gallus gallus α-RyR gene (homologous to RyR 1) that could be associated with PSE (pale, soft and exudative) meat. Because RyR genes are over 100,000 bp long and code for proteins with about 5000 amino acids, primers were designed to amplify a fragment of hotspot region 2, a region with a high density of mutations in other species. Total blood DNA was extracted from 50 birds, 25 that had PSE meat and 25 normal chickens. The DNA samples were amplified by PCR, cloned, sequenced, and used to identify single nucleotide polymorphisms (SNPs). The amplified fragment of α-RyR was 604 nucleotides in length; 181 nucleotides were similar to two exons from a hypothetical turkey cDNA sequence for α-RyR. A non-synonymous nucleotide substitution (G/A) was identified in at least one of the three sequenced clones obtained from nine animals, six PSE (HAL+) birds and three normal (HAL-) birds; they were heterozygous for this mutation.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleA new single nucleotide polymorphism in the ryanodine gene of chicken skeletal muscle.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2017-07-13T18:48:40Zpt_BR
dc.subject.thesagroMutaçãopt_BR
dc.subject.thesagroGenéticapt_BR
dc.subject.thesagroCarnept_BR
dc.subject.thesagroQualidadept_BR
dc.subject.nalthesaurusMutationpt_BR
dc.subject.nalthesaurusGeneticspt_BR
dc.subject.nalthesaurusMeat qualitypt_BR
riaa.ainfo.id941757pt_BR
riaa.ainfo.lastupdate2017-07-13pt_BR
dc.contributor.institutionADRIANA A. DROVAL, UEL; ELISEU BINNECK, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; FERNANDA G. PAIÃO, UEL; A. OBA, UEL; ALEXANDRE LIMA NEPOMUCENO, SRI; MASSAMI SHIMOKOMAKI, UTFPR.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

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