Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/943053
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dc.contributor.authorSILVA, M. R.
dc.contributor.authorGUIMARÃES, M. D. C.
dc.contributor.authorOLIVEIRA, V. M. de
dc.contributor.authorCOSTA, R. R.
dc.contributor.authorABI-ZAID, K. C. F.
dc.contributor.authorARAUJO, F. R.
dc.contributor.authorSOUZA, G. N. de
dc.contributor.authorREIS, R. A.
dc.contributor.authorHYLARIO, S. M.
dc.contributor.authorAMARAL, C. M.
dc.contributor.authorSUFFYS, P. N.
dc.contributor.authorROCHA, A. DA SILVA
dc.date.accessioned2025-06-05T13:49:02Z-
dc.date.available2025-06-05T13:49:02Z-
dc.date.created2012-12-19
dc.date.issued2012
dc.identifier.citationIn: CONGRESSO INTERNACIONAL DO LEITE, 11.; WORKSHOP DE POLÍTICAS PÚBLICAS, 11.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 12., 2012, Goiânia. Anais... Juiz de Fora: Embrapa Gado de Leite, 2012.
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/943053-
dc.descriptionA cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC.
dc.language.isospa
dc.rightsopenAccess
dc.subjectPseudogene oxyR
dc.subjectGene pnA
dc.titleIdentification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil.
dc.typeArtigo em anais e proceedings
dc.subject.thesagroTuberculose
dc.subject.nalthesauruszoonoses
riaa.ainfo.id943053
riaa.ainfo.lastupdate2025-06-05
dc.contributor.institutionMARCIO ROBERTO SILVA, CNPGL; UNIVERSIDADE FEDERAL DE MINAS GERAIS; VANIA MARIA DE OLIVEIRA, CNPGL; UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FLABIO RIBEIRO ARAUJO, CNPGC; GUILHERME NUNES DE SOUZA, CNPGL; FUNDAÇÃO OSWALDO CRUZ; FUNDAÇÃO OSWALDO CRUZ.
Appears in Collections:Artigo em anais de congresso (CNPGL)

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