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dc.contributor.authorSOUZA, V. dept_BR
dc.contributor.authorFILHO, A. N.pt_BR
dc.contributor.authorMELO, P. de C.pt_BR
dc.contributor.authorFERNANDES, G. M.pt_BR
dc.contributor.authorFERRAUDO, A. S.pt_BR
dc.contributor.authorCONDE, S. de O.pt_BR
dc.contributor.authorFOGAÇA JÚNIOR, F. A.pt_BR
dc.date.accessioned2013-02-20T11:11:11Zpt_BR
dc.date.available2013-02-20T11:11:11Zpt_BR
dc.date.created2013-02-20pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationBrazilian Journal of Microbiology, São Paulo, v. 43, n. 4, p. 1646-1650, out./dez. 2012.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/950132pt_BR
dc.descriptionAbstract: The epidemiological relationships between isolated Staphylococcus aureus strains in milk samples of dairy cows, reagent to California Mastitis Test, individual and group milk was demonstrated in different sites of the production fluxogram, in 12 milk-producing farms in the Gameleira region, municipality of Sacramento MG Brazil, so that localization and transmission modes may be identified. Two hundred and forty-four strains out of 446 samples collected at several sites were isolated and bio-chemically characterized as coagulase-positive staphylococcus. Specific chromosome DNA fragment of the species Staphylococcus aureus was amplified to 106 strains and 103 underwent (PFGE). Samples? collection sites with the highest isolation frequency of Staphylococcus aureus strains comprised papillary ostia (31.1%), CMT-reagent cow milk (21.7%), mechanical milking machines? insufflators (21,7%), milk in milk pails (6.6%) and the milk in community bulk tanks (5.6%). Genetic heterogeneity existed among the isolated 103 Staphylococcus aureus strains, since 32 different pulse-types were identified. Pulse-type 1 had the highest similarity among the isolated strains within the different sites of the milk-production fluxogram. Highest occurrence of pulsetype 1 isolates of Staphylococcus aureus strains was reported in samples collected from the papillary ostia (10.6%), followed by milk samples from CMT-reagent dairy cows (5.8%) and mechanical milking machine insufflators (3.8%). The above shows the relevance of these sites in the agents? transmission mechanism within the context of the farms investigated.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectBrasilpt_BR
dc.subjectMinas Geraispt_BR
dc.subjectPFGEpt_BR
dc.subjectBulk tankpt_BR
dc.subjectPCRpt_BR
dc.titleMolecular epidemiology of Staphylococcus aureus isolates at different sites in the milk producing dairy farms.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-04-20T11:11:11Zpt_BR
dc.subject.thesagroStaphylococcus aureuspt_BR
dc.subject.thesagroMamitept_BR
dc.subject.thesagroVaca leiteirapt_BR
dc.subject.thesagroDoença animalpt_BR
dc.subject.nalthesaurusMastitispt_BR
dc.subject.nalthesaurusDairy cowspt_BR
dc.subject.nalthesaurusMammary gland diseasespt_BR
dc.subject.nalthesaurusBrazilpt_BR
riaa.ainfo.id950132pt_BR
riaa.ainfo.lastupdate2015-04-20pt_BR
dc.contributor.institutionVIVIANE DE SOUZA, CNPC; Antonio Nader Filho, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP; Poliana de Castro Melo, UNESP - Jaboticabal, SP; Guilherme Moraes Ferraudo, UNESP - Jaboticabal, SP.; Antônio Sérgio Ferraudo, UNESP - Jaboticabal, SP; Sandra de Oliveira Conde, UNESP - Jaboticabal, SP; Flavio Augusto Fogaça Junior, UNESP - Jaboticabal, SP.pt_BR
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