Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/952212
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dc.contributor.authorCARVALHO, S. L. dept_BR
dc.contributor.authorSILVA, F. N. dapt_BR
dc.contributor.authorZANARDO, L. G.pt_BR
dc.contributor.authorALMEIDA, A. M. R.pt_BR
dc.contributor.authorZERBINI, F. M.pt_BR
dc.contributor.authorCARVALHO, C. M.pt_BR
dc.date.accessioned2013-03-05T11:11:11Zpt_BR
dc.date.available2013-03-05T11:11:11Zpt_BR
dc.date.created2013-03-05pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationTropical Plant Patology, Brasília, DF, v. 38, n. 1, p. 49-54, Jan.-Feb. 2013.pt_BR
dc.identifier.issn1982-5676pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/952212pt_BR
dc.descriptionCowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass spectrometer analyses. A rabbit was immunized with increasing amounts of the recombinant protein. The specificity and sensitivity of the antiserum was demonstrated by Western blot and indirect ELISA assays. The polyclonal antisera raised against recombinant coat protein proved to be a reliable tool for CpMMV detection.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleProduction of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-03-25T11:11:11Zpt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroVíruspt_BR
dc.subject.thesagroSojapt_BR
dc.subject.nalthesaurusPlant diseases and disorderspt_BR
dc.subject.nalthesaurusCowpea mild mottle viruspt_BR
dc.subject.nalthesaurusSoybeanspt_BR
riaa.ainfo.id952212pt_BR
riaa.ainfo.lastupdate2013-03-25pt_BR
dc.contributor.institutionSILVIA L. DE CARVALHO, UFV; FÁBIO N. DA SILVA, UFV; LARISSA G. ZANARDO, UFV; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSO; F. MURILO ZERBINI, UFV; CLAUDINE M. CARVALHO, UFV.pt_BR
Aparece nas coleções:Artigo em periódico indexado (CNPSO)

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