Please use this identifier to cite or link to this item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/957083
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dc.contributor.authorCARVALHO, G. A. B. dept_BR
dc.contributor.authorBATISTA, J. S. S.pt_BR
dc.contributor.authorMARCELINO-GUIMARÃES, F. C.pt_BR
dc.contributor.authorNASCIMENTO, L. C. dopt_BR
dc.contributor.authorHUNGRIA, M.pt_BR
dc.date.accessioned2013-05-03T11:11:11Zpt_BR
dc.date.available2013-05-03T11:11:11Zpt_BR
dc.date.created2013-05-03pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationBMC Genomics, v. 14, n. 153, 2013.pt_BR
dc.identifier.issn1471-2164pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/957083pt_BR
dc.descriptionBiological nitrogen fixation in root nodules is a process of great importance to crops of soybean [Glycine max (L.) Merr.], as it may provide the bulk of the plant?s needs for nitrogen. Legume nodulation involves several complex steps and, although studied for many decades, much remains to be understood. Results: This research aimed at analyzing the global expression of genes in soybean roots of a Brazilian cultivar (Conquista) inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. To achieve this, we used the suppressive subtractive hybridization (SSH) technique combined with Illumina sequencing. The subtractive library (non-inoculated x inoculated) of soybean roots resulted in 3,210 differentially expressed transcripts at 10 days after inoculation were studied. The data were grouped according to the ontologies of the molecular functions and biological processes. Several classes of genes were confirmed as related to N2 fixation and others were reported for the first time. During nodule formation, a higher percentage of genes were related to primary metabolism, cell-wall modifications and the antioxidant defense system. Putative symbiotic functions were attributed to some of these genes for the first time.pt_BR
dc.languageenpt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectNodulação - sojapt_BR
dc.titleTranscriptional analysis of genes involved in nodulation in soybean roots inoculated with Bradyrhizobium japonicum strain CPAC 15.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2014-03-11T11:11:11Zpt_BR
dc.format.extent211 p.pt_BR
riaa.ainfo.id957083pt_BR
riaa.ainfo.lastupdate2014-03-11pt_BR
dc.identifier.doi10.1186/1471-2164-14-153pt_BR
dc.contributor.institutionGESIELE ALMEIDA BARROS DE CARVALHO; JESIANE S. S. BATISTA; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; LEANDRO COSTA DO NASCIMENTO; MARIANGELA HUNGRIA DA CUNHA, CNPSO.pt_BR
Appears in Collections:Artigo em periódico indexado (CNPSO)

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