Título:	Molecular typing of mastitis-causing Staphylococcus aureus isolated from heifers and cows.
Autoria:	CASTELANI, L. SANTOS, A. F. S. MIRANDA, M. dos S. ZAFALON, L. F. POZZI, C. R. ARCARO, J. R. P.
Afiliação:	LÍVIA CASTELANI, Instituto de Zootecnia; ALINE FRANCIELE SILVA SANTOS, Instituto de Zootecnia; MARIANA DOS SANTOS MIRANDA, Instituto de Zootecnia; LUIZ FRANCISCO ZAFALON, CPPSE; CLAUDIA RODRIGUES POZZI, Instituto de Zootecnia; JULIANA RODRIGUES POZZI ARCARO, Instituto de Zootecnia.
Ano de publicação:	2013
Referência:	International Journal of Molecular Sciences, v. 14, p. 4326-4333, 2013.
Conteúdo:	Staphylococcus aureus is among the main etiologic agents of bovine mastitis. A total of 83 isolates of S. aureus from mammary glands of primiparous heifers were collected in the prepartum, calving and during lactation. For lactating cows, a total of 27 isolates of S. aureus from mammary glands were collected during lactation. The samples were taken in two dairy farms located in Sao Paulo State, Brazil. The highest frequency of S. aureus isolation in heifers was at the end of lactation. Strains were typified through Pulsed-field gel electrophoresis (PFGE) and grouped according to patterns of restriction enzyme SmaI. PFGE generated seven clonal profiles that were grouped into three different lineages, with the LA lineage being predominant and identified in heifers, as well as in the cows from the two regions studied. It was concluded that the cows showed a significant source of dispersion of S. aureus. At the first lactation the heifers were infected by the same clonal profiles of S. aureus which were isolated from multiparous lactating cows. The heifers were infected during milking over the months of lactation.
NAL Thesaurus:	epidemiology heifers
Palavras-chave:	PFGE
Digital Object Identifier:	10.3390/ijms14024326
Tipo do material:	Artigo de periódico
Acesso:	openAccess
Aparece nas coleções:	Artigo em periódico indexado (CPPSE)