Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/960427
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dc.contributor.authorMELO, P. de C.pt_BR
dc.contributor.authorFERREIRA, L. M.pt_BR
dc.contributor.authorNADER FILHO, A.pt_BR
dc.contributor.authorZAFALON, L. F.pt_BR
dc.contributor.authorVICENTE, H. I. G.pt_BR
dc.contributor.authorSOUZA, V. dept_BR
dc.date.accessioned2013-06-24T11:11:11Zpt_BR
dc.date.available2013-06-24T11:11:11Zpt_BR
dc.date.created2013-06-24pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationBrazilian Journal of Microbiology, São Paulo, v. 44, n. 1, p. 119-124, 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/960427pt_BR
dc.descriptionAbstract: Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and the presence of the icaA and icaD genes. The results revealed that 85% of the isolates tested produced slime on the Congo red agar, 98.9% of the isolates produced biofilms in vitro by adhering to sterile 96-well "U" bottom polystyrene tissue culture plates, and 95.7% of the isolates carried the icaA and icaD genes. The results of the phenotypic tests for biofilm formation were compared with those of the molecular analysis, and the sensitivity and specificity of the Congo red agar test were 88.9% and 100%, respectively, while those of the microtitre plate test were 100% and 25%, respectively. When the phenotypic methods for the detection of biofilm producers, namely growth on microtitre plates and Congo red agar, were compared, the sensitivity and specificity were 86% and 100%, respectively. Therefore, growth on Congo red agar and the microtitre plate test are methods that could be used to determine whether an isolate has the potential for biofilm production.pt_BR
dc.language.isoporpt_BR
dc.rightsopenAccesspt_BR
dc.subjectMastite bovinapt_BR
dc.subjectPhenotypicpt_BR
dc.subjectMolecular analysispt_BR
dc.subjectBiofilmspt_BR
dc.titleComparison of methods for the detection of biofilm formation by Staphylococcus aureus isolated from bovine subclinical mastitis.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-04-15T11:11:11Zpt_BR
dc.subject.thesagroBovinopt_BR
dc.subject.thesagroDoença animalpt_BR
dc.subject.thesagroMamitept_BR
dc.subject.thesagroStaphylococcus aureuspt_BR
dc.subject.thesagroBiofilmept_BR
dc.subject.thesagroEstirpept_BR
dc.subject.nalthesaurusBovine mastitispt_BR
dc.subject.nalthesaurusMammary gland diseasespt_BR
riaa.ainfo.id960427pt_BR
riaa.ainfo.lastupdate2015-04-15pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S1517-83822013005000031pt_BR
dc.contributor.institutionPoliana de Castro Melo, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) - Jaboticabal, SP, Brazil.; Luciano Menezes Ferreira, UNESP - Jaboticabal, SP, Brazil; Antônio Nader Filho, UNESP - Jaboticabal, SP, Brazil; LUIZ FRANCISCO ZAFALON, CPPSE; Hinig Isa Godoy Vicente, Secretaria da Agricultura do Estado de São Paulo, Jaboticabal, SP, Brazil; VIVIANE DE SOUZA, CNPC.pt_BR
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