Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963146
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dc.contributor.authorSAWAZAKI, H. E.pt_BR
dc.contributor.authorSA, L. A. N. dept_BR
dc.contributor.authorGONÇALVES, C. R. N. C. B.pt_BR
dc.contributor.authorVEIGA, R. F. A.pt_BR
dc.contributor.authorCOLOMBO, C. A.pt_BR
dc.date.accessioned2013-08-01T11:11:11Zpt_BR
dc.date.available2013-08-01T11:11:11Zpt_BR
dc.date.created2013-08-01pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationInternacional Research Journal of Plant Science, Sapele, v. 4, n. 3, p. 76-83, 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/963146pt_BR
dc.descriptionAbstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of primers designed for orange rust was more specific. The pair of primers designed for scald, apparently was not able to distinguish only bacteria of the genus Erwinia, whereas the primers described in the literature were observed amplify others genera, such as Xanthomonas, Pseudomonas, Erwinia, Stenotrophomona and Pantoea. The three strains of the fungus curvularia, found in sugarcane, resembled more to the species C.cymbopogonis, C.geniculata, C.sp. Of the 28 primer pairs tested, the best were shown for each of the disease-causing agent.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLeaf scaldpt_BR
dc.subjectPCRpt_BR
dc.titleMolecular diagnosis optimization of virus, bacteria and fungi in sugarcane.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-08-02T11:11:11Zpt_BR
dc.subject.thesagroCana de açúcarpt_BR
dc.subject.thesagroDoença de plantapt_BR
dc.subject.thesagroDiagnósticopt_BR
dc.subject.thesagroBactériapt_BR
dc.subject.thesagroVíruspt_BR
dc.subject.thesagroFungopt_BR
dc.subject.thesagroEscaldadurapt_BR
dc.subject.thesagroFerrugem alaranjadapt_BR
dc.subject.nalthesaurusPlant diseases and disorderspt_BR
dc.subject.nalthesaurusSugarcanept_BR
dc.subject.nalthesaurusRatoon stunting diseasept_BR
dc.subject.nalthesaurusScald diseasespt_BR
dc.subject.nalthesaurusSugarcane yellow leaf viruspt_BR
dc.subject.nalthesaurusSugarcane streak viruspt_BR
dc.subject.nalthesaurusFijiviruspt_BR
dc.subject.nalthesaurusSmut diseasespt_BR
dc.subject.nalthesaurusRust diseasespt_BR
dc.subject.nalthesaurusCurvulariapt_BR
dc.subject.nalthesaurusPolymerase chain reactionpt_BR
riaa.ainfo.id963146pt_BR
riaa.ainfo.lastupdate2013-08-02pt_BR
dc.contributor.institutionHAIKO ENOK SAWAZAKI, IAC; LUIZ ALEXANDRE NOGUEIRA DE SA, CNPMA; CASSIARA REGINA NOVENTA CORREA BARBOSA GONÇALVES, CTC; RENATO FERRAS DE ARRUDA VEIGA, IAC; CARLOS AUGUSTO COLOMBO, IAC.pt_BR
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