Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/973494
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dc.contributor.authorSILVA, M. S. dapt_BR
dc.contributor.authorMONTEIRO, J. P.pt_BR
dc.contributor.authorNUNES, V. S.pt_BR
dc.contributor.authorVASCONCELOS, E. J.pt_BR
dc.contributor.authorPEREZ, A. M.pt_BR
dc.contributor.authorFREITAS JÚNIOR, L. de H.pt_BR
dc.contributor.authorELIAS, M. C.pt_BR
dc.date.accessioned2013-12-11T11:11:11Zpt_BR
dc.date.available2013-12-11T11:11:11Zpt_BR
dc.date.created2013-12-11pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationPLoS ONE, San Francisco, v. 8, n. 11, e81397, Nov. 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/973494pt_BR
dc.descriptionAbstract: Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLeishmaniose tegumentarpt_BR
dc.subjectDivisão celularpt_BR
dc.subjectCinetoplastopt_BR
dc.subjectReplicaçãopt_BR
dc.subjectNúcleo celularpt_BR
dc.subjectReplicationpt_BR
dc.titleLeishmania amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation during cell cycle.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2015-04-14T11:11:11Zpt_BR
dc.subject.thesagroLeishmaniosept_BR
dc.subject.thesagroDoença animalpt_BR
dc.subject.thesagroFisiologiapt_BR
dc.subject.nalthesaurusLeishmaniapt_BR
dc.subject.nalthesaurusCell divisionpt_BR
dc.subject.nalthesaurusKinetoplastidapt_BR
dc.subject.nalthesaurusDNA probespt_BR
dc.subject.nalthesaurusPhysiologypt_BR
dc.subject.nalthesaurusLeishmania amazonensispt_BR
dc.subject.nalthesauruscell nucleuspt_BR
dc.subject.nalthesaurusleishmaniasispt_BR
riaa.ainfo.id973494pt_BR
riaa.ainfo.lastupdate2015-04-14pt_BR
dc.identifier.doi10.1371/journal.pone.0081397pt_BR
dc.contributor.institutionMarcelo Santos da Silva, Instituto de Biociências, Universidade Estadual Paulista (UNESP) - Botucatu, São Paulo, Brazilpt_BR
dc.contributor.institutionJOMAR PATRICIO MONTEIRO, CNPCeng
dc.contributor.institutionVinícius Santana Nunes, UNESP - Botucatu, São Paulo, Brazileng
dc.contributor.institutionElton José Vasconcelos, Seattle Biomedical Research Institute - Seattle, Washington, United States of Americaeng
dc.contributor.institutionArina Marina Perez, UNESP - Botucatu, São Paulo, Brazileng
dc.contributor.institutionLúcio de Holanda Freitas-Júnior, Laboratório Nacional de Biociências (LNBio), Centro Nacional de Pesquisa em Energia e Materiais - Campinas, São Paulo, Brazileng
dc.contributor.institutionMaria Carolina Elias, Center of Toxins, Immune Response and Cell Signaling (CeTICS), Instituto Butantan - São Paulo, SP, Brazil.eng
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