Use este identificador para citar ou linkar para este item: http://www.alice.cnptia.embrapa.br/alice/handle/doc/973974
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dc.contributor.authorROLIM, A. A. S. G.pt_BR
dc.contributor.authorYANO, S. A. C.pt_BR
dc.contributor.authorSPECHT, A.pt_BR
dc.contributor.authorANDRADE, C. G. T. de J.pt_BR
dc.contributor.authorSOSA-GOMEZ, D. R.pt_BR
dc.date.accessioned2013-12-16T22:30:00Z-
dc.date.available2013-12-16T22:30:00Z-
dc.date.created2013-12-16pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationAnnals of the Entomological Society of America, v. 106, n. 5, p. 643-651, 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/973974pt_BR
dc.descriptionThe most important species of Lepidoptera that attack soybean, in descending order of importance, are Chrysodeixis includens (Walker), Anticarsia gemmatalis Hübner, Spodoptera cosmioides (Walker), Spodoptera eridania (Stoll), and Spodoptera albula (Walker). In addition, Rachiplusia nu (Guenée) is one of the most common Plusiinae in the southern region of Brazil, encompassing the states of Paraná, Santa Catarina, and Rio Grande do Sul. The correct identification of these species is essential for choosing the appropriate control measures, as they differ in their susceptibility to insecticides and biological control agents. To distinguish Lepidoptera species commonly found in Brazil, the eggshells of all these species were morphologically characterized by scanning electron microscopy. Diagnostic characters for differentiating species include shape of rosette petals, number of primary cells, number of secondary cells, number of ribs, and rosette diameter. Also, a molecular diagnostic method using polymerase chain reaction and restriction fragment length polymorphism (PCR?RFLP) analysis was developed to distinguish noctuid species commonly found in Brazil. A 658-bp region of the mitochondrial DNA cytochrome oxidase subunit I gene was amplified using PCR and then sequenced. The five Lepidoptera species were distinguished by restriction enzymes Bpm I and MboI. RFLPs produced by Bpm I endonuclease were useful to discriminate species from within Spodoptera (S. cosmioides, S. eridania, and S. albula) and R. nu from C. includens. However, Bpm I did not digest the amplicons from S. eridania and C. includens, which were discriminated by RFLP patterns produced by the restriction enzyme MboI. PCR-RFLP can be performed in a short period, and it is useful to distinguish the most important Brazilian Lepidoptera soybean pests.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectLepidoteropt_BR
dc.titleMorphological and molecular characterization of the eggs of some Noctuid Species associated with soybean in Brazil.pt_BR
dc.typeArtigo de periódicopt_BR
dc.date.updated2013-12-16T22:30:00Zpt_BR
dc.subject.thesagroSojapt_BR
dc.subject.thesagroPraga de plantapt_BR
dc.subject.thesagroPhytophthorapt_BR
dc.subject.thesagroPseudoplusia Includenspt_BR
dc.subject.thesagroSpodoptera Eridaniapt_BR
dc.subject.nalthesaurusLepidopterapt_BR
dc.subject.nalthesaurusRachiplusiapt_BR
dc.subject.nalthesaurusSpodoptera albulapt_BR
dc.subject.nalthesaurusPlant pestspt_BR
dc.subject.nalthesaurusSoybeanspt_BR
dc.subject.nalthesaurusBrazilpt_BR
dc.subject.nalthesaurusSpodoptera cosmioidespt_BR
riaa.ainfo.id973974pt_BR
riaa.ainfo.lastupdate2013-12-16pt_BR
dc.identifier.doi10.1603/AN13049pt_BR
dc.contributor.institutionADRIAN AUGUSTO SOSA GOMEZ ROLIM, ESALQ - USP; SILVIA AKIMI CAVAGUCHI YANO, CNPSo; ALEXANDRE SPECHT, CPAC; CELIA GUADALUPE TARDELLI DE JESUS ANDRADE, UEL; DANIEL RICARDO SOSA GOMEZ, CNPSO.pt_BR
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