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dc.contributor.authorCINTRA, L. C.pt_BR
dc.contributor.authorZERLOTINI, A.pt_BR
dc.contributor.authorLOBO, F. P.pt_BR
dc.contributor.authorSILVA, F. R. dapt_BR
dc.contributor.authorGIACHETTO, P. F.pt_BR
dc.contributor.authorKUSER-FALCÃO, P. R.pt_BR
dc.contributor.authorSILVA, L. O. C. dapt_BR
dc.contributor.authorEGITO, A. A. dopt_BR
dc.contributor.authorSIQUEIRA, F.pt_BR
dc.contributor.authorSILVA, N. M. A. dapt_BR
dc.contributor.authorPAIVA, S. R.pt_BR
dc.contributor.authorYAMAGISHI, M. E. B.pt_BR
dc.contributor.authorCAETANO, A. R.pt_BR
dc.date.accessioned2014-01-02T11:11:11Zpt_BR
dc.date.available2014-01-02T11:11:11Zpt_BR
dc.date.created2014-01-02pt_BR
dc.date.issued2013pt_BR
dc.identifier.citationIn: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. [Abstracts...]. [S.l.: s.n.], 2013.pt_BR
dc.identifier.urihttp://www.alice.cnptia.embrapa.br/alice/handle/doc/974758pt_BR
dc.descriptionBos indicus cattle breeds have been extensively used for dairy and beef production in tropical climates and present several natural adaptations to biotic and abiotic stresses found in these regions of the world. As breeder associations stride towards incorporating genomic tools into ongoing genetic evaluations and breeding programs to improve productivity and beef and milk quality traits, a (B. indicus) genome assembly represents an essential tool which will be vital to help identify and understand the underlying genetic variations that distinguish taurine and indicine cattle, which have diverged >250,000 years ago. DNA obtained from semen from a Nelore bull born in 1987, with an estimated cumulative inbreeding coefficient of 29.4%, and that can be traced to animals imported from India, was used to produce 100bp paired-end sequences from short (300 and 700bp) and long insert (3, 5 and 10 kbp) libraries, with an Illumina HiSeq platform. A total of 120Gbp were sequenced, corresponding to 45x raw coverage of the genome. The SOAP de novo assembler was used to build contigs and scaffolding. Several parameters sets were evaluated to obtain the best assembly based on the number of scaffolds, number of bases in scaffolds, N50, and total gap length. The best assembly obtained so far contains 2.7Gbp, 15,103 scaffolds with N50 of 649Kbp, and 756Mbp of gaps. Current results are being used to target additional sequencing of specific libraries to improve scaffold assembly. In addition, different sequencing technologies are also under evaluation for generating additional data to improve sequence assembly quality before comparisons with the reference (B. taurus) sequence are performed.pt_BR
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectSequenciamento de genomapt_BR
dc.subjectBioinformáticapt_BR
dc.titleSequencing and de novo genome assembly of a nelore (Bos indicus) bull.pt_BR
dc.typeResumo em anais e proceedingspt_BR
dc.date.updated2020-01-22T11:11:11Zpt_BR
dc.subject.thesagroBos Indicuspt_BR
dc.subject.nalthesaurusGenome assemblypt_BR
dc.subject.nalthesaurusBioinformaticspt_BR
dc.subject.nalthesaurusNellorept_BR
dc.description.notesPôster 0522.pt_BR
dc.format.extent2Não paginado.pt_BR
riaa.ainfo.id974758pt_BR
riaa.ainfo.lastupdate2020-01-22 -02:00:00pt_BR
dc.contributor.institutionLEANDRO CARRIJO CINTRA, CNPTIA; ADHEMAR ZERLOTINI NETO, CNPTIA; FRANCISCO PEREIRA LOBO, CNPTIA; FELIPE RODRIGUES DA SILVA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; LUIZ OTAVIO CAMPOS DA SILVA, CNPGC; ANDREA ALVES DO EGITO, CNPGC; FABIANE SIQUEIRA, CNPGC; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; SAMUEL REZENDE PAIVA, Cenargen; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; ALEXANDRE RODRIGUES CAETANO, CENARGEN.pt_BR
Aparece nas coleções:Resumo em anais de congresso (CNPTIA)

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